Bioaugmentation of the thermophilic anaerobic biodegradation of cellulose and corn stover

Orsolya Strang, Norbert Ács, Roland Wirth, Gergely Maróti, Zoltán Bagi, G. Rákhely, K. Kovács

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Two stable, thermophilic mixed cellulolytic consortia were enriched from an industrial scale biogas fermenter. The two consortia, marked as AD1 and AD2, were used for bioaugmentation in laboratory scale batch reactors. They enhanced the methane yield by 22–24%. Next generation sequencing method revealed the main orders being Thermoanaerobacterales and Clostridiales and the predominant strains were Thermoanaerobacterium thermosaccharolyticum, Caldanaerobacter subterraneus, Thermoanaerobacter pseudethanolicus and Clostridium cellulolyticum. The effect of these strains, cultivated in pure cultures, was investigated with the aim of reconstructing the defined cellulolytic consortium. The addition of the four bacterial strains and their mixture to the biogas fermenters enhanced the methane yield by 10–11% but it was not as efficient as the original communities indicating the significant contribution by members of the enriched communities present in low abundance.

Original languageEnglish
Pages (from-to)104-113
Number of pages10
JournalAnaerobe
Volume46
DOIs
Publication statusPublished - Aug 1 2017

Fingerprint

Biofuels
Methane
Cellulose
Zea mays
Clostridium cellulolyticum
Thermoanaerobacterium
Thermoanaerobacter
Clostridiales

Keywords

  • Biaugmentation
  • Biogas
  • Clostridiales
  • Thermoanaerobacterales
  • Thermophilic cellulolytic consortia

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Bioaugmentation of the thermophilic anaerobic biodegradation of cellulose and corn stover. / Strang, Orsolya; Ács, Norbert; Wirth, Roland; Maróti, Gergely; Bagi, Zoltán; Rákhely, G.; Kovács, K.

In: Anaerobe, Vol. 46, 01.08.2017, p. 104-113.

Research output: Contribution to journalArticle

Strang, Orsolya ; Ács, Norbert ; Wirth, Roland ; Maróti, Gergely ; Bagi, Zoltán ; Rákhely, G. ; Kovács, K. / Bioaugmentation of the thermophilic anaerobic biodegradation of cellulose and corn stover. In: Anaerobe. 2017 ; Vol. 46. pp. 104-113.
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