Bioartificial heart: A human-sized porcine model - The way ahead

Alexander Weymann, Nikhil Prakash Patil, Anton Sabashnikov, Philipp Jungebluth, Sevil Korkmaz, Shiliang Li, Gabor Veres, P. Soós, Roland Ishtok, Nicole Chaimow, Ines Pätzold, Natalie Czerny, Carsten Schies, Bastian Schmack, Aron Frederik Popov, André Rüdiger Simon, Matthias Karck, G. Szabó

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background: A bioartificial heart is a theoretical alternative to transplantation or mechanical left ventricular support. Native hearts decellularized with preserved architecture and vasculature may provide an acellular tissue platform for organ regeneration. We sought to develop a tissue-engineered whole-heart neoscaffold in human-sized porcine hearts. Methods: We decellularized porcine hearts (n = 10) by coronary perfusion with ionic detergents in a modified Langendorff circuit. We confirmed decellularization by histology, transmission electron microscopy and fluorescence microscopy, quantified residual DNA by spectrophotometry, and evaluated biomechanical stability with ex-vivo left-ventricular pressure/ volume studies, all compared to controls. We then mounted the decellularized porcine hearts in a bioreactor and reseeded them with murine neonatal cardiac cells and human umbilical cord derived endothelial cells (HUVEC) under simulated physiological conditions. Results: Decellularized hearts lacked intracellular components but retained specific collagen fibers, proteoglycan, elastin and mechanical integrity; quantitative DNA analysis demonstrated a significant reduction of DNA compared to controls (82.6±3.2 ng DNA/mg tissue vs. 473.2±13.4 ng DNA/mg tissue, p

Original languageEnglish
Article numbere111591
JournalPLoS One
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 3 2014

Fingerprint

Swine
heart
swine
DNA
Tissue
elastin
umbilical cord
Histology
Elastin
proteoglycans
Umbilical Cord
Spectrophotometry
Fluorescence microscopy
Endothelial cells
Bioreactors
Ventricular Pressure
Proteoglycans
bioreactors
fluorescence microscopy
Transmission Electron Microscopy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Weymann, A., Patil, N. P., Sabashnikov, A., Jungebluth, P., Korkmaz, S., Li, S., ... Szabó, G. (2014). Bioartificial heart: A human-sized porcine model - The way ahead. PLoS One, 9(11), [e111591]. https://doi.org/10.1371/journal.pone.0111591

Bioartificial heart : A human-sized porcine model - The way ahead. / Weymann, Alexander; Patil, Nikhil Prakash; Sabashnikov, Anton; Jungebluth, Philipp; Korkmaz, Sevil; Li, Shiliang; Veres, Gabor; Soós, P.; Ishtok, Roland; Chaimow, Nicole; Pätzold, Ines; Czerny, Natalie; Schies, Carsten; Schmack, Bastian; Popov, Aron Frederik; Simon, André Rüdiger; Karck, Matthias; Szabó, G.

In: PLoS One, Vol. 9, No. 11, e111591, 03.11.2014.

Research output: Contribution to journalArticle

Weymann, A, Patil, NP, Sabashnikov, A, Jungebluth, P, Korkmaz, S, Li, S, Veres, G, Soós, P, Ishtok, R, Chaimow, N, Pätzold, I, Czerny, N, Schies, C, Schmack, B, Popov, AF, Simon, AR, Karck, M & Szabó, G 2014, 'Bioartificial heart: A human-sized porcine model - The way ahead', PLoS One, vol. 9, no. 11, e111591. https://doi.org/10.1371/journal.pone.0111591
Weymann A, Patil NP, Sabashnikov A, Jungebluth P, Korkmaz S, Li S et al. Bioartificial heart: A human-sized porcine model - The way ahead. PLoS One. 2014 Nov 3;9(11). e111591. https://doi.org/10.1371/journal.pone.0111591
Weymann, Alexander ; Patil, Nikhil Prakash ; Sabashnikov, Anton ; Jungebluth, Philipp ; Korkmaz, Sevil ; Li, Shiliang ; Veres, Gabor ; Soós, P. ; Ishtok, Roland ; Chaimow, Nicole ; Pätzold, Ines ; Czerny, Natalie ; Schies, Carsten ; Schmack, Bastian ; Popov, Aron Frederik ; Simon, André Rüdiger ; Karck, Matthias ; Szabó, G. / Bioartificial heart : A human-sized porcine model - The way ahead. In: PLoS One. 2014 ; Vol. 9, No. 11.
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