BioArena: An unlimited possibility of biochemical interactions in the adsorbent layer after chromatographic separation

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23 Citations (Scopus)

Abstract

The BioArena system, which integrates the modern technique and biological results of bioautography with layer liquid chromatography is especially suitable for investigating biochemical interactions. Formaldehyde (HCHO) and its reaction products play a crucial role in the antibiotic activity of trans-resveratrol and other molecules - when HCHO-capturing molecules are used in culture media the antimicrobial activity of antibiotic-like compounds decreases substantially. HCHO and hydrogen peroxide are present as normal endogenous compounds in cells, so there is a possibility of interaction in which singlet oxygen (1O2) and excited HCHO can be formed. The 1O2 can oxidize water molecules and so H2O 3 can be formed, from which, by disproportion, among other reactions, ozone (O3) also can be formed in the chromatographic spots. Elimination of HCHO and/or O3 from the spots results in a decrease in the antiproliferative effect.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalJournal of Planar Chromatography - Modern TLC
Volume21
Issue number1
DOIs
Publication statusPublished - Feb 2008

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Adsorbents
Anti-Bacterial Agents
Singlet Oxygen
Molecules
Ozone
Liquid Chromatography
Hydrogen Peroxide
Formaldehyde
Culture Media
Liquid chromatography
Reaction products
Water
resveratrol

Keywords

  • BioArena
  • Bioautography
  • Formaldehyde
  • OPLC
  • Ozone
  • TLC

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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abstract = "The BioArena system, which integrates the modern technique and biological results of bioautography with layer liquid chromatography is especially suitable for investigating biochemical interactions. Formaldehyde (HCHO) and its reaction products play a crucial role in the antibiotic activity of trans-resveratrol and other molecules - when HCHO-capturing molecules are used in culture media the antimicrobial activity of antibiotic-like compounds decreases substantially. HCHO and hydrogen peroxide are present as normal endogenous compounds in cells, so there is a possibility of interaction in which singlet oxygen (1O2) and excited HCHO can be formed. The 1O2 can oxidize water molecules and so H2O 3 can be formed, from which, by disproportion, among other reactions, ozone (O3) also can be formed in the chromatographic spots. Elimination of HCHO and/or O3 from the spots results in a decrease in the antiproliferative effect.",
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author = "E. Tyih{\'a}k and E. Mincsovics and G. K{\'a}tay and Z. Kir{\'a}ly-V{\'e}ghely and A. M{\'o}ricz and P. Ott",
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AU - Mincsovics, E.

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AU - Móricz, A.

AU - Ott, P.

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