Bioactivity guided isolation of phytoestrogenic compounds from Cyclopia genistoides by the pER8: GUS reporter system

O. Roza, W. C. Lai, I. Zupkó, J. Hohmann, N. Jedlinszki, F. R. Chang, D. Csupor, J. N. Eloff

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The popular South African herbal tea, honeybush, is made from several . Cyclopia species (family: . Fabaceae), amongst them . Cyclopia genistoides. Phytoestrogenic potential of . C. genistoides. has been recently reported, however bioactivity-guided isolation of compounds with estrogenic activity has not yet been performed.A transgenic plant system, . Arabidopsis thaliana pER8:GUS, was used to assay the estrogen-like activity of . C. genistoides. The quantitative determination of the active compounds in the fermented and non-fermented plant material was performed by HPLC. Subsequent bioactivity-guided fractionation led to the isolation of genistein, naringenin, isoliquiritigenin, luteolin, helichrysin B and 5,7,3',5'-tetrahydroxyflavanone, four of them first reported in the genus.Helichrysin B, naringenin and 5,7,3',5'-tetrahydroxyflavanone differed in quantity in the fermented and unfermented herbs, the fermented plant material contained two compounds with substantial estrogenic-like activity in higher concentration (naringenin and 5,7,3',5'-tetrahydroxyflavanone), whereas the less active helichrysin B was more abundant in the unfermented herb. The fractions as well as compounds inhibited the growth of human cancer cell lines A2780 and T47D.These results underline the phytoestrogenic activity of . C. genistoides and support the rationale to the fermentation process.

Original languageEnglish
JournalSouth African Journal of Botany
DOIs
Publication statusAccepted/In press - Nov 6 2015

Fingerprint

naringenin
herbs
Cyclopia
herbal tea
estrogenic properties
human growth
luteolin
genistein
estrogens
Fabaceae
transgenic plants
fractionation
Arabidopsis thaliana
fermentation
cell lines
Cyclopia genistoides
assays

Keywords

  • Cyclopia
  • Genistein
  • Luteolin
  • Menopause
  • PER8:GUS
  • Phytoestrogen

ASJC Scopus subject areas

  • Plant Science

Cite this

Bioactivity guided isolation of phytoestrogenic compounds from Cyclopia genistoides by the pER8 : GUS reporter system. / Roza, O.; Lai, W. C.; Zupkó, I.; Hohmann, J.; Jedlinszki, N.; Chang, F. R.; Csupor, D.; Eloff, J. N.

In: South African Journal of Botany, 06.11.2015.

Research output: Contribution to journalArticle

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