Bioactivities of Anastasia Black (Russian sweet pepper)

Yoshiaki Shirataki, Masami Kawase, Hiroshi Sakagami, Hideki Nakashima, Satoru Tani, Toru Tanaka, Yoshitaka Sohara, Zsuzsanna Schelz, Joseph Molnar, Noboru Motohashi

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7 Citations (Scopus)


Anastasia Black (Russian sweet pepper) of Capsicum annuum L. var. angulosum Mill. (Solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of twenty-three fractions by silica gel or octadecylsilane (ODS; C18) column chromatography. These extracts and fractions were investigated for their cytotoxicity, anti-human immunodeficiency virus (HIV), anti-Helicobacter pylori (H. pylori), urease inhibition and multidrug resistance (MDR) reversal activity. Some fractions of hexane and acetone extracts showed higher cytotoxic activity against three human oral tumor cell lines (squamous cell carcinoma HSC-2, HSC-3, submandibular gland carcinoma HSG) than against three normal human oral cells (gingival fibroblast HGF, pulp cell HPC, periodontal ligament fibroblast HPLF), suggesting a tumor-specific cytotoxic activity. No fractions displayed anti-HIV activity, but some hydrophobic fractions showed higher anti-H. pylori activity, urease inhibition activity and MDR reversal activity. The higher MDR activity of these fractions against MDR gene-transfected L5178 mouse lymphoma T cells may possibly be due to their higher content of carotene or polyphenol. These data suggest that Anastasia Black should be further investigated as a potent supplement for cancer chemotherapy.

Original languageEnglish
Pages (from-to)1991-1999
Number of pages9
JournalAnticancer research
Issue number3 B
Publication statusPublished - May 1 2005


  • Anti-HIV activity
  • Anti-Helicobacter pylori activity
  • Capsicum annuum L. var. angulosum Mill
  • Cytotoxicity
  • Extracts
  • MDR reversal
  • Urease inhibition

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Shirataki, Y., Kawase, M., Sakagami, H., Nakashima, H., Tani, S., Tanaka, T., Sohara, Y., Schelz, Z., Molnar, J., & Motohashi, N. (2005). Bioactivities of Anastasia Black (Russian sweet pepper). Anticancer research, 25(3 B), 1991-1999.