Binding studies with recombinant human serum albumin obtained by expression of a synthetic gene in yeast. Stereoselective binding and allosteric interaction with benzodiazepine and coumarin ligands

Ilona Fitos, Júlia Visy, András Simoncsits

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Abstract

The specific ligand binding ability of recombinant human serum albumin produced in yeast using the synthetic gene was studied by affinity Chromatographic method. It was found that synthetic protein possesses those stereoselective binding and binding interactions for several chiral benzodiazepine and coumarin compounds which are characteristic of the natural human serum albumin, suggesting identical tertiary structures.

Original languageEnglish
Pages (from-to)1159-1163
Number of pages5
JournalBiochemical Pharmacology
Volume46
Issue number7
DOIs
Publication statusPublished - Oct 5 1993

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ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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