Binding sites of photoreceptor-specific antibodies COS-1, OS-2 and AO

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The chicken red-sensitive cone visual pigment iodosin) and several synthetic peptides of cone and rod tisuar pigments were used to find the binding sites of our photoreceptor-specific antibodies with immunocytochemistry. The ability of iodopsin to block immunolabeling with monoclonal antibodies COS-1 and OS-2 furnished direct evidence that both antibodies are specific to visual pigments. lmmunocytochemistry on whole-mount retinas with and without detergent, as well as electron microscopic labeling of cone photoreceptor membranes revealed the binding sites of COS-1 and OS-2 to be on the cytoplasmic side of the membrane. By testing several synthetic peptides, mainly from the C-terminal region of the cone visual pigments, we found that the domain consisting of the last 6 amino acids of the human red/green-, and the chicken red-sensitive cone pigments completely blocked immunolabeling with COS-1, while the sequence consisting of the last 12 amino acids of the human blue cone pigment was effective to block the binding of OS-2. Both monoclonals can be regarded therefore C-terminal specific antibodies. OS-2 was found to bind to the dark-adapted photopigment more strongly than to the light-adapted one. The binding of the polyclonal rhodopsin antibody AO was almost entirely inhibited by the N-terminal synthetic peptide of bovine rhodopsin indicating that this antibody binds primarily to the N-terminal domain of rhodopsin in a tissue environment.

Original languageEnglish
Pages (from-to)935-944
Number of pages10
JournalCurrent Eye Research
Volume12
Issue number10
DOIs
Publication statusPublished - 1993

Fingerprint

Binding Sites
Retinal Pigments
Rhodopsin
Antibodies
lissamine rhodamine B
Chickens
Retinal Cone Photoreceptor Cells
Amino Acids
Peptides
Vertebrate Photoreceptor Cells
Detergents
Retina
Immunohistochemistry
Monoclonal Antibodies
Cell Membrane
Electrons
Light
Membranes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Binding sites of photoreceptor-specific antibodies COS-1, OS-2 and AO. / Röhlich, P.; Szél, A.

In: Current Eye Research, Vol. 12, No. 10, 1993, p. 935-944.

Research output: Contribution to journalArticle

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