Binding sites for inositol trisphosphate in the bovine adrenal cortex

Albert J. Baukal, Gaetan Guillemette, Ronald Rubin, András Spät, Kevin J. Catt

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Abstract

Binding sites for inositol trisphosphate (IP3) have been identified in bovine adrenal cortex, employing [32P]IP3 prepared from human erythrocytes radiolabeled with [32P]ATP. IP3 was bound to adrenal microsomes with high affinity (Kd = 5 nM) and low capacity (186 fmol/mg protein). During kinetic studies, half-maximal binding was reached in less than one min at 4°C, and dissociation was even more rapid with t 1 2 of about 10 sec. [32P]IP2 showed no binding to the microsomal sites, which represent putative receptors at which IP3 acts to elevate intracellular calcium concentration during the actions of peptide hormones such as angiotensin II.

Original languageEnglish
Pages (from-to)532-538
Number of pages7
JournalBiochemical and biophysical research communications
Volume133
Issue number2
DOIs
Publication statusPublished - Dec 17 1985

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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