Binding of the enzymes of fatty acid β-oxidation and some related enzymes to pig heart inner mitochondrial membrane

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The binding of crotonase (enoyl-CoA hydratase), β-hydroxyacyl-CoA dehydrogenase, β-ketothiolase, succinyl-CoA transferase, and carnitine acetyltransferase to inner mitochondrial membranes, mitoplasts, intact mitochondria, erythrocyte membranes, and phosphatidylcholine liposomes was studied. Succinyl-CoA transferase does not bind to any of these membranes. Carnitine acetyltransferase, on the other hand, bind to all of these membranes. The enzymes of fatty acid β-oxidation, thiolase, crotonase, and β-hydroxyacyl-CoA dehydrogenase binds to inner membrane, but not to liposomes. The binding shows a moderate dependence on ionic strength (2-200 mM) and pH (6.5-8). These data indicate the possibility of an organization of the enzymes of fatty acid β-oxidation on the inner mitochondrial membrane, but do not support the idea of an organization of the enzymes of ketone body catabolism.

Original languageEnglish
Pages (from-to)8748-8752
Number of pages5
JournalJournal of Biological Chemistry
Volume259
Issue number14
Publication statusPublished - Jan 1 1984

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Binding of the enzymes of fatty acid β-oxidation and some related enzymes to pig heart inner mitochondrial membrane'. Together they form a unique fingerprint.

  • Cite this