The binding of crotonase (enoyl-CoA hydratase), β-hydroxyacyl-CoA dehydrogenase, β-ketothiolase, succinyl-CoA transferase, and carnitine acetyltransferase to inner mitochondrial membranes, mitoplasts, intact mitochondria, erythrocyte membranes, and phosphatidylcholine liposomes was studied. Succinyl-CoA transferase does not bind to any of these membranes. Carnitine acetyltransferase, on the other hand, bind to all of these membranes. The enzymes of fatty acid β-oxidation, thiolase, crotonase, and β-hydroxyacyl-CoA dehydrogenase binds to inner membrane, but not to liposomes. The binding shows a moderate dependence on ionic strength (2-200 mM) and pH (6.5-8). These data indicate the possibility of an organization of the enzymes of fatty acid β-oxidation on the inner mitochondrial membrane, but do not support the idea of an organization of the enzymes of ketone body catabolism.
|Number of pages||5|
|Journal||Journal of Biological Chemistry|
|Publication status||Published - Jan 1 1984|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology