Binding of new cationic porphyrin-tetrapeptide conjugates to nucleoprotein complexes

Ádám Orosz, G. Mező, Levente Herényi, Jan Habdas, Zs. Majer, Beata Myśliwa-Kurdziel, Katalin Tóth, G. Csík

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ongoing research on DNA binding of cationic porphyrin derivatives and their conjugates is a subject of growing interest because of their possible DNA binding and demonstrated biological properties. In this study nucleoprotein binding of tri-cationic meso-tri(4-N-methylpyridyl)-mono-(4-carboxyphenyl) porphyrin (TMPCP) and tetrapeptides conjugated TMPCP (TMPCP-4P) and bi-cationic meso-5,10-bis(4-N-methylpyridyl)-15,20-di-(4-carboxyphenyl)porphyrin (BMPCP-4P2) was investigated with comprehensive spectroscopic methods. The key observation is that tetrapeptide-conjugates of cationic porphyrins with two or three positive charges bind to encapsidated DNA in T7 phage nucleoprotein complex. The binding modes were analyzed by fluorescent energy transfer, fluorescent life time and CD measurements. Intercalative binding is most feasible when tricationic ligands complex with DNA, especially when it is in close connection with protein capsid. It was found that larger ligand BMPCP-4P2 binds externally to encapsidated T7 DNA, and complex externally as well as by intercalation when the DNA accommodate to relaxed B-conformation. In the case of TMPCP and TMPCP-4P the intercalation is the predominant binding form both in nucleoprotein (NP) and preheated complexes. Further, melting experiments revealed that bound porphyrins do not influence the capsid stability or protein-DNA interactions, but efficiently stabilize the double helical structure of DNA without respect to binding form. A good correlation was found between porphyrin/base pair ration and DNA strand separation temperature.

Original languageEnglish
Pages (from-to)14-23
Number of pages10
JournalBiophysical Chemistry
Volume177-178
DOIs
Publication statusPublished - Jul 2013

Fingerprint

Nucleoproteins
Porphyrins
DNA
Intercalation
Ligands
Bacteriophage T7
Bacteriophages
Protein Stability
Capsid
Energy Transfer
Capsid Proteins
Base Pairing
Energy transfer
Freezing
Conformations
Melting
Observation
Derivatives
Temperature

Keywords

  • Absorption melting
  • Cationic porphyrin
  • Fluorescence spectroscopy
  • Induced ECD-signal
  • Nucleoprotein-porphyrin binding
  • Porphyrin-peptide conjugate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Organic Chemistry

Cite this

Binding of new cationic porphyrin-tetrapeptide conjugates to nucleoprotein complexes. / Orosz, Ádám; Mező, G.; Herényi, Levente; Habdas, Jan; Majer, Zs.; Myśliwa-Kurdziel, Beata; Tóth, Katalin; Csík, G.

In: Biophysical Chemistry, Vol. 177-178, 07.2013, p. 14-23.

Research output: Contribution to journalArticle

Orosz, Ádám ; Mező, G. ; Herényi, Levente ; Habdas, Jan ; Majer, Zs. ; Myśliwa-Kurdziel, Beata ; Tóth, Katalin ; Csík, G. / Binding of new cationic porphyrin-tetrapeptide conjugates to nucleoprotein complexes. In: Biophysical Chemistry. 2013 ; Vol. 177-178. pp. 14-23.
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AU - Majer, Zs.

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