Binding of mycoplasmas to solid phase adsorbents

Susan Szathmáry, Nandani Rajapakse, Ibolya Székely, E. Pitlik, Judit Bíró, Noémi Erdei, L. Stipkovits

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The capture of mycoplasmas (M. hominis, M. buccale, M. fermentans, M. bovis, M. synoviae, M. gallisepticum and M. arthritidis) based on lipid structures and adhesion molecules present in the mycoplasmal membrane was tested using different chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow and SulfEtox). All of the resins efficiently reduced mycoplasma concentrations in Phosphate Buffered Saline (PBS) and in Fetal Bovine Serum (FBS) by 3-8 logs in a few minutes. This technology could be used for removing mycoplasmas from tissue culture components such as serum, and for concentrating mycoplasmas in vaccine production.

Original languageEnglish
Pages (from-to)299-307
Number of pages9
JournalActa veterinaria Hungarica
Volume53
Issue number3
DOIs
Publication statusPublished - Aug 26 2005

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Keywords

  • Chromatography resin
  • Mycoplasma

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Szathmáry, S., Rajapakse, N., Székely, I., Pitlik, E., Bíró, J., Erdei, N., & Stipkovits, L. (2005). Binding of mycoplasmas to solid phase adsorbents. Acta veterinaria Hungarica, 53(3), 299-307. https://doi.org/10.1556/AVet.53.2005.3.3