Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions

A. Spät, G. L. Lukács, I. Eberhardt, L. Kiesel, B. Runnebaum

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Bovine anterior-pituitary microsomal fractions exhibit high-affinity, saturable and reversible binding of inositol 1,4,5-[32P]trisphosphate; 50% of the labelled ligand is displaced by 3.5 nM-inositol 1,4,5-trisphosphate, 0.5 μM-inositol 1,4-bisphosphate and 10 μM-ATP. Inositol 1,4,5-trisphosphate induces the release of Ca2+ from the microsomal vesicles (half-maximal effect at 290 nM), and its action is potentiated by inositol tetrakisphosphate (half-maximal effect at 4 μM).

Original languageEnglish
Pages (from-to)493-496
Number of pages4
JournalBiochemical Journal
Volume244
Issue number2
Publication statusPublished - 1987

Fingerprint

Inositol 1,4,5-Trisphosphate
Inositol Phosphates
Inositol
Adenosine Triphosphate
Ligands
inositol 1,4-bis(phosphate)

ASJC Scopus subject areas

  • Biochemistry

Cite this

Spät, A., Lukács, G. L., Eberhardt, I., Kiesel, L., & Runnebaum, B. (1987). Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions. Biochemical Journal, 244(2), 493-496.

Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions. / Spät, A.; Lukács, G. L.; Eberhardt, I.; Kiesel, L.; Runnebaum, B.

In: Biochemical Journal, Vol. 244, No. 2, 1987, p. 493-496.

Research output: Contribution to journalArticle

Spät, A, Lukács, GL, Eberhardt, I, Kiesel, L & Runnebaum, B 1987, 'Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions', Biochemical Journal, vol. 244, no. 2, pp. 493-496.
Spät, A. ; Lukács, G. L. ; Eberhardt, I. ; Kiesel, L. ; Runnebaum, B. / Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions. In: Biochemical Journal. 1987 ; Vol. 244, No. 2. pp. 493-496.
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