Binding of CCCTC-binding factor in vivo to the region located between Rep* and the C promoterof Epstein-Barr virus is unaffected by CpG methylation and does not correlate with Cp activity

Daniel Salamon, Ferenc Banati, Anita Koroknai, Mate Ravasz, Kalman Szenthe, Zoltan Bathori, Agnes Bakos, Hans Helmut Niller, Hans Wolf, Janos Minarovits

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Abstract

In this study, the binding of the insulator protein CCCTC-binding factor (CTCF) to the region located between Rep and the C promoter (Cp) of Epstein-Barr virus (EBV) was analysed using chromatin immunoprecipitation and in vivo footprinting. CTCF binding was found to be independent of Cp usage in cell lines corresponding to the major EBV latency types. Bisulfite sequencing and an electrophoretic mobility-shift assay (using methylated and unmethylated probes) revealed that CTCF binding was insufficient to induce local CpG demethylation in certain cell lines and was unaffected by CpG methylation in the region between Rep and Cp. In addition, CTCF binding to the latency promoter, Qp, did not correlate with Qp activity.

Original languageEnglish
Pages (from-to)1183-1189
Number of pages7
JournalJournal of General Virology
Volume90
Issue number5
DOIs
Publication statusPublished - Jun 29 2009

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ASJC Scopus subject areas

  • Virology

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