Binding of cationic porphyrin to isolated and encapsidated viral DNA analyzed by comprehensive spectroscopic methods

Kristóf Zupán, Levente Herényi, Katalin Tóth, Zs. Majer, G. Csík

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The complexation of tetrakis(4-N-methylpyridyl)porphyrin (TMPyP) with free and encapsidated DNA Of T7 bacteriophage was investigated. To identify binding modes and relative concentrations of bound TMPyP forms, the porphyrin absorption spectra at various base pair/porphyrin ratios were analyzed. Spectral decomposition, fluorescent lifetime, and circular dichroism measurements proved the presence of two main binding types of TMPyP, e.g., external binding and intercalation both in free and in encapsidated DNA. Optical melting studies revealed that TMPyP increases the strand separation temperature of both free and native phage DNA and does not change the phase transition temperature of phage capsid proteins. From these findings we concluded that TMPyP binding does not influence the protein structure and/or the protein-DNA interaction. A combined analysis of absorption spectra and fluorescence decay curves made possible the determination of concentrations of free, externally bound, and intercalated porphyrin. As a perspective, our results facilitate a qualitative analysis of the TMPyP binding process at various experimental conditions.

Original languageEnglish
Pages (from-to)9151-9159
Number of pages9
JournalBiochemistry
Volume43
Issue number28
DOIs
Publication statusPublished - Jul 20 2004

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Porphyrins
Viral DNA
Bacteriophages
DNA
Absorption spectra
Bacteriophage T7
Transition Temperature
Phase Transition
Capsid Proteins
Intercalation
Circular Dichroism
Complexation
Base Pairing
Freezing
Spectrum Analysis
Proteins
Melting
Fluorescence
Phase transitions
Decomposition

ASJC Scopus subject areas

  • Biochemistry

Cite this

Binding of cationic porphyrin to isolated and encapsidated viral DNA analyzed by comprehensive spectroscopic methods. / Zupán, Kristóf; Herényi, Levente; Tóth, Katalin; Majer, Zs.; Csík, G.

In: Biochemistry, Vol. 43, No. 28, 20.07.2004, p. 9151-9159.

Research output: Contribution to journalArticle

Zupán, Kristóf ; Herényi, Levente ; Tóth, Katalin ; Majer, Zs. ; Csík, G. / Binding of cationic porphyrin to isolated and encapsidated viral DNA analyzed by comprehensive spectroscopic methods. In: Biochemistry. 2004 ; Vol. 43, No. 28. pp. 9151-9159.
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