Binding mode analysis and enrichment studies on homology models of the human histamine H4 receptor

Róbert Kiss, Béla Noszál, Ákos Rácz, András Falus, Dániel Eros, György M. Keseru

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Ligand-supported homology models of the human histamine H4 receptor (hH4R) were developed based on the crystal structure of bovine rhodopsin and different known H4 ligands (histamine, OUP-16, JNJ7777120). Enrichment tests were performed to analyze whether our hH4R models can select known actives from random decoys. The impact of receptor conformation and the effect of different sets of random decoys, docking methods (FlexX, FlexX-Pharm) and scoring functions (FlexX-Score, D-Score, PMF-Score, G-Score, ChemScore) were investigated. We found that two agonists (histamine and OUP-16) form complementary interactions with Asp94 (3.32), Glu182 (5.46) and Thr323 (6.55), whereas JNJ7777120 interacts with Asp94 (3.32) and Glu182 (5.46) only. These results suggest a role of Thr323 (6.55) in ligand binding and presumably also in receptor activation. The models optimized in the presence of an agonist (histamine) and an antagonist (JNJ7777120) were compared in more detail. We conclude that the ligand used in the model building process can significantly influence the efficacy of virtual screening.

Original languageEnglish
Pages (from-to)1059-1070
Number of pages12
JournalEuropean Journal of Medicinal Chemistry
Volume43
Issue number5
DOIs
Publication statusPublished - May 1 2008

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Keywords

  • Binding site
  • Docking
  • Enrichment
  • H4 receptor
  • Histamine
  • Scoring

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

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