Binding interaction of gamma aminobutyric acid A and B receptors in cell culture

J. Kardos, Llona Kovacs

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have examined the effects of 0.1 mM baclofen and pertussis toxin treatment on gamma aminobutyric acid A (GABAA) receptor binding in whole cells and in membrane of cells from cerebellar primary culture. Baclofen, a GABAB receptor Hgand, caused a marked decrease in the bicuculline sensitive (GABAA) binding of [3H]muscimol on the whole cells, but failed to inhibit specific [3H]muscimol binding to cells pretreated with pertussis toxin. The effect of baclofen was only seen in whole cells and not in cell membranes. These findings suggest that activation of GABAB receptor reduces GABAA binding sites through a GTP binding protein.

Original languageEnglish
Pages (from-to)541-543
Number of pages3
JournalNeuroReport
Volume2
Issue number9
Publication statusPublished - 1991

Fingerprint

Baclofen
gamma-Aminobutyric Acid
Muscimol
GABA Receptors
Cell Culture Techniques
Pertussis Toxin
Cell Membrane
Primary Cell Culture
Bicuculline
GTP-Binding Proteins
Binding Sites

Keywords

  • Baclofen
  • Cerebellum
  • Pertussis toxin
  • Primary culture
  • Rat
  • [<sup>3</sup>H]muscimol binding

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Binding interaction of gamma aminobutyric acid A and B receptors in cell culture. / Kardos, J.; Kovacs, Llona.

In: NeuroReport, Vol. 2, No. 9, 1991, p. 541-543.

Research output: Contribution to journalArticle

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