Binding crevice for TT-232 in a homology model of type 1 somatostatin receptor

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7 Citations (Scopus)

Abstract

Somatostatin receptor type 1 was modelled based on the atomic structure of bovine rhodopsin. Possible ways of binding interaction between somatostatin receptor type 1 and TT-232, a cycloheptapeptide analogue of somatostatin with broad therapeutic potential, were analysed by molecular docking. The twelve TT-232 conformations, obtained by NMR measurements in H2O-D 2O mixture, were similar, disclosing a consensus backbone conformation. Several residues interacting with TT-232, such as Val133, Asp137 (helix 3), Arg197 (helix 4), Phe287, Gln291, Asn294 (helix 6), Ser305, and Tyr313 (helix 7), were found. In accordance, in vitro binding experiments indicated high-affinity binding of TT-232 to 125I labelled somatostatin sites in brain membranes. The single binding crevice obtained by docking may allow the design and discovery of new peptidomimetics of TT-232 in the future.

Original languageEnglish
Pages (from-to)1059-1064
Number of pages6
JournalBiochemical and biophysical research communications
Volume316
Issue number4
DOIs
Publication statusPublished - Apr 16 2004

Keywords

  • Homology model
  • NMR
  • Somatostatin receptor
  • TT-232 binding
  • TT-232 conformation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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