Binding assays with molecularly imprinted polymers - Why do they work?

Tímea Pap, G. Horvai

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The design of homologous displacement ligand binding assays based on molecularly imprinted polymers (MIP) is discussed in terms of the MIP adsorption isotherm. It is shown that only MIPs having a binding isotherm with varying slope are suitable for the assay, but there is no need to interpret the isotherm in terms of site affinity and population. One can calculate the calibration plot of the binding assay from the isotherm and vice versa.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume804
Issue number1
DOIs
Publication statusPublished - May 5 2004

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Isotherms
Assays
Polymers
Calibration
Adsorption
Ligands
Adsorption isotherms
Population

Keywords

  • Binding assays
  • Molecularly imprinted polymers

ASJC Scopus subject areas

  • Biochemistry

Cite this

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