Bigfoot

A new family of MITE elements characterized from the Medicago genus

Bénédicte Charrier, Fabrice Foucher, É. Kondorosi, Yves D'Aubenton-Carafa, Claude Thermes, Adam Kondorosi, Pascal Ratet

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We have characterized from the legume plant Medicago a new family of miniature inverted-repeat transposable elements (MITE), called the Bigfoot transposable elements. Two of these insertion elements are present only in a single allele of two different M. sativa genes. Using a PCR strategy we have isolated 19 other Bigfoot elements from the M. sativa and M. truncatula genomes. They differ from the previously characterized MITEs by their sequence, a target site of 9 bp and a partially clustered genomic distribution, in addition, we show that they exhibit a significantly stable secondary structure. These elements may represent up to 0.1% of the genome of the outcrossing Medicago sativa but are present at a reduced copy number in the genome of the autogamous M. truncatula plant, revealing major differences in the genome organization of these two plants.

Original languageEnglish
Pages (from-to)431-441
Number of pages11
JournalPlant Journal
Volume18
Issue number4
DOIs
Publication statusPublished - May 1999

Fingerprint

miniature inverted repeat transposable elements
Medicago
DNA Transposable Elements
new family
Genome
genome
transposons
Medicago sativa
Medicago truncatula
outcrossing
Fabaceae
legumes
Alleles
alleles
genomics
Polymerase Chain Reaction
Legendary Creatures
Genes
genes

ASJC Scopus subject areas

  • Plant Science

Cite this

Charrier, B., Foucher, F., Kondorosi, É., D'Aubenton-Carafa, Y., Thermes, C., Kondorosi, A., & Ratet, P. (1999). Bigfoot: A new family of MITE elements characterized from the Medicago genus. Plant Journal, 18(4), 431-441. https://doi.org/10.1046/j.1365-313X.1999.00469.x

Bigfoot : A new family of MITE elements characterized from the Medicago genus. / Charrier, Bénédicte; Foucher, Fabrice; Kondorosi, É.; D'Aubenton-Carafa, Yves; Thermes, Claude; Kondorosi, Adam; Ratet, Pascal.

In: Plant Journal, Vol. 18, No. 4, 05.1999, p. 431-441.

Research output: Contribution to journalArticle

Charrier, B, Foucher, F, Kondorosi, É, D'Aubenton-Carafa, Y, Thermes, C, Kondorosi, A & Ratet, P 1999, 'Bigfoot: A new family of MITE elements characterized from the Medicago genus', Plant Journal, vol. 18, no. 4, pp. 431-441. https://doi.org/10.1046/j.1365-313X.1999.00469.x
Charrier B, Foucher F, Kondorosi É, D'Aubenton-Carafa Y, Thermes C, Kondorosi A et al. Bigfoot: A new family of MITE elements characterized from the Medicago genus. Plant Journal. 1999 May;18(4):431-441. https://doi.org/10.1046/j.1365-313X.1999.00469.x
Charrier, Bénédicte ; Foucher, Fabrice ; Kondorosi, É. ; D'Aubenton-Carafa, Yves ; Thermes, Claude ; Kondorosi, Adam ; Ratet, Pascal. / Bigfoot : A new family of MITE elements characterized from the Medicago genus. In: Plant Journal. 1999 ; Vol. 18, No. 4. pp. 431-441.
@article{f23bcafd0d714fa5bb7ced6cfe3aae6a,
title = "Bigfoot: A new family of MITE elements characterized from the Medicago genus",
abstract = "We have characterized from the legume plant Medicago a new family of miniature inverted-repeat transposable elements (MITE), called the Bigfoot transposable elements. Two of these insertion elements are present only in a single allele of two different M. sativa genes. Using a PCR strategy we have isolated 19 other Bigfoot elements from the M. sativa and M. truncatula genomes. They differ from the previously characterized MITEs by their sequence, a target site of 9 bp and a partially clustered genomic distribution, in addition, we show that they exhibit a significantly stable secondary structure. These elements may represent up to 0.1{\%} of the genome of the outcrossing Medicago sativa but are present at a reduced copy number in the genome of the autogamous M. truncatula plant, revealing major differences in the genome organization of these two plants.",
author = "B{\'e}n{\'e}dicte Charrier and Fabrice Foucher and {\'E}. Kondorosi and Yves D'Aubenton-Carafa and Claude Thermes and Adam Kondorosi and Pascal Ratet",
year = "1999",
month = "5",
doi = "10.1046/j.1365-313X.1999.00469.x",
language = "English",
volume = "18",
pages = "431--441",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Bigfoot

T2 - A new family of MITE elements characterized from the Medicago genus

AU - Charrier, Bénédicte

AU - Foucher, Fabrice

AU - Kondorosi, É.

AU - D'Aubenton-Carafa, Yves

AU - Thermes, Claude

AU - Kondorosi, Adam

AU - Ratet, Pascal

PY - 1999/5

Y1 - 1999/5

N2 - We have characterized from the legume plant Medicago a new family of miniature inverted-repeat transposable elements (MITE), called the Bigfoot transposable elements. Two of these insertion elements are present only in a single allele of two different M. sativa genes. Using a PCR strategy we have isolated 19 other Bigfoot elements from the M. sativa and M. truncatula genomes. They differ from the previously characterized MITEs by their sequence, a target site of 9 bp and a partially clustered genomic distribution, in addition, we show that they exhibit a significantly stable secondary structure. These elements may represent up to 0.1% of the genome of the outcrossing Medicago sativa but are present at a reduced copy number in the genome of the autogamous M. truncatula plant, revealing major differences in the genome organization of these two plants.

AB - We have characterized from the legume plant Medicago a new family of miniature inverted-repeat transposable elements (MITE), called the Bigfoot transposable elements. Two of these insertion elements are present only in a single allele of two different M. sativa genes. Using a PCR strategy we have isolated 19 other Bigfoot elements from the M. sativa and M. truncatula genomes. They differ from the previously characterized MITEs by their sequence, a target site of 9 bp and a partially clustered genomic distribution, in addition, we show that they exhibit a significantly stable secondary structure. These elements may represent up to 0.1% of the genome of the outcrossing Medicago sativa but are present at a reduced copy number in the genome of the autogamous M. truncatula plant, revealing major differences in the genome organization of these two plants.

UR - http://www.scopus.com/inward/record.url?scp=0033136549&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033136549&partnerID=8YFLogxK

U2 - 10.1046/j.1365-313X.1999.00469.x

DO - 10.1046/j.1365-313X.1999.00469.x

M3 - Article

VL - 18

SP - 431

EP - 441

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 4

ER -