Bigfoot: A new family of MITE elements characterized from the Medicago genus

Bénédicte Charrier, Fabrice Foucher, Eva Kondorosi, Yves D'Aubenton-Carafa, Claude Thermes, Adam Kondorosi, Pascal Ratet

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Abstract

We have characterized from the legume plant Medicago a new family of miniature inverted-repeat transposable elements (MITE), called the Bigfoot transposable elements. Two of these insertion elements are present only in a single allele of two different M. sativa genes. Using a PCR strategy we have isolated 19 other Bigfoot elements from the M. sativa and M. truncatula genomes. They differ from the previously characterized MITEs by their sequence, a target site of 9 bp and a partially clustered genomic distribution, in addition, we show that they exhibit a significantly stable secondary structure. These elements may represent up to 0.1% of the genome of the outcrossing Medicago sativa but are present at a reduced copy number in the genome of the autogamous M. truncatula plant, revealing major differences in the genome organization of these two plants.

Original languageEnglish
Pages (from-to)431-441
Number of pages11
JournalPlant Journal
Volume18
Issue number4
DOIs
Publication statusPublished - May 1 1999

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ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Charrier, B., Foucher, F., Kondorosi, E., D'Aubenton-Carafa, Y., Thermes, C., Kondorosi, A., & Ratet, P. (1999). Bigfoot: A new family of MITE elements characterized from the Medicago genus. Plant Journal, 18(4), 431-441. https://doi.org/10.1046/j.1365-313X.1999.00469.x