Bidirectional enhancing activities between human T cell leukemia-lymphoma virus type I and human cytomegalovirus in human term syncytiotrophoblast cells cultured in vitro

F. Tóth, G. Aboagye-Mathiesen, J. Szabó, X. Liu, P. Mosborg-Petersen, L. Kiss, H. Hager, M. Zdravkovic, I. Andirko, J. Aranyosi, P. Ebbesen

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Abstract

The syncytiotrophoblast layer of the human placenta has an important role in limiting transplacental viral spread from mother to fetus. Human cytomegalovirus (HCMV) is capable of establishing a latent infection in syncytiotrophoblast cells, with restriction of gene expression to immediate-early and early proteins. We analyzed the extent of replication of human T cell leukemia-lymphoma virus type I (HTLV-I) in human term syncytiotrophoblasts infected with HTLV-I alone or coinfected with HTLV-I and HCMV. Although syncytiotrophoblasts could be infected with cell-free HTLV-I, no viral protein expression was found in the singly infected cells. On the contrary, coinfection of the cells with HTLV-I and HCMV resulted in simultaneous replication of both viruses. Bidirectional enhancing activities between HTLV-I and HCMV were mediated primarily by the Tax and immediate-early proteins, respectively. The stimulatory effect of HTLV-I Tax on HCMV replication appeared to be mediated partly by tumor necrosis factor β and transforming growth factor β-1. We observed formation of pseudotypes with HTLV-I nucleocapsids within HCMV envelopes, whereas HCMV was not pseudotyped by HTLV-I envelopes in dually infected syncytiotrophoblast cells. Our data suggest that in vivo dual infection of syncytiotrophoblast cells with HTLV-I and HCMV may facilitate the transplacental transmission of both viruses.

Original languageEnglish
Pages (from-to)1495-1507
Number of pages13
JournalAIDS Research and Human Retroviruses
Volume11
Issue number12
Publication statusPublished - 1995

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Deltaretrovirus
Human T-lymphotropic virus 1
Trophoblasts
Cytomegalovirus
Cultured Cells
In Vitro Techniques
Immediate-Early Proteins
Nucleocapsid
Transforming Growth Factors
Viral Proteins
Virus Replication
Infection
Coinfection
Placenta
Fetus
Tumor Necrosis Factor-alpha
Viruses

ASJC Scopus subject areas

  • Immunology
  • Virology

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Bidirectional enhancing activities between human T cell leukemia-lymphoma virus type I and human cytomegalovirus in human term syncytiotrophoblast cells cultured in vitro. / Tóth, F.; Aboagye-Mathiesen, G.; Szabó, J.; Liu, X.; Mosborg-Petersen, P.; Kiss, L.; Hager, H.; Zdravkovic, M.; Andirko, I.; Aranyosi, J.; Ebbesen, P.

In: AIDS Research and Human Retroviruses, Vol. 11, No. 12, 1995, p. 1495-1507.

Research output: Contribution to journalArticle

Tóth, F, Aboagye-Mathiesen, G, Szabó, J, Liu, X, Mosborg-Petersen, P, Kiss, L, Hager, H, Zdravkovic, M, Andirko, I, Aranyosi, J & Ebbesen, P 1995, 'Bidirectional enhancing activities between human T cell leukemia-lymphoma virus type I and human cytomegalovirus in human term syncytiotrophoblast cells cultured in vitro', AIDS Research and Human Retroviruses, vol. 11, no. 12, pp. 1495-1507.
Tóth, F. ; Aboagye-Mathiesen, G. ; Szabó, J. ; Liu, X. ; Mosborg-Petersen, P. ; Kiss, L. ; Hager, H. ; Zdravkovic, M. ; Andirko, I. ; Aranyosi, J. ; Ebbesen, P. / Bidirectional enhancing activities between human T cell leukemia-lymphoma virus type I and human cytomegalovirus in human term syncytiotrophoblast cells cultured in vitro. In: AIDS Research and Human Retroviruses. 1995 ; Vol. 11, No. 12. pp. 1495-1507.
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