Beyond the physico-chemical barrier: Glycerol and xylitol markedly yet differentially alter gene expression profiles and modify signalling pathways in human epidermal keratinocytes

Edit Páyer, Judit Szabó-Papp, Lídia Ambrus, Attila Gábor Szöllősi, Mónika Andrási, Shabtay Dikstein, L. Kemény, I. Juhász, A. Szegedi, T. Bíró, Attila Oláh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Polyols (e.g. glycerol, xylitol) are implicated as moisturizers of the skin and other epithelial tissues. However, we lack information about their exact cellular mechanisms and their effects on the gene expression profiles. Therefore, in this study, we aimed at investigating the effects of glycerol and xylitol on human epidermal keratinocytes. The polyols (identical osmolarities; xylitol: 0.0045%-0.45%; glycerol: 0.0027%-0.27%) did not alter cellular viability or intracellular calcium concentration. However, they exerted differential effects on the expression of certain genes and signalling pathways. Indeed, both polyols up-regulated the expression of filaggrin, loricrin, involucrin and occludin; yet, xylitol exerted somewhat more profound effects. Moreover, while both polyols stimulated the MAPK pathway, only xylitol induced the activation-dependent translocation of protein kinase Cδ, a key promoter of epidermal differentiation. Finally, in various keratinocyte inflammation models, both polyols (albeit with different efficacies) exerted anti-inflammatory effects. Taken together, these data strongly suggest that glycerol and xylitol differentially modulate expressions of multiple genes and activities of signalling pathways in epidermal keratinocytes. Thus, our findings invite clinical trials to explore the applicability and the impact of a combined glycerol-xylitol therapy in the management of various skin conditions.

Original languageEnglish
Pages (from-to)280-284
Number of pages5
JournalExperimental Dermatology
Volume27
Issue number3
DOIs
Publication statusPublished - Mar 1 2018

Keywords

  • glycerol
  • inflammation
  • keratinocyte
  • keratinocyte differentiation
  • xylitol

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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