Beyond homopolymer errors: A systematic investigation of nanopore-based DNA sequencing characteristics using HLA-DQA2

Péter Sárközy, Viktor Molnár, Dóra Fogl, C. Szalai, Péter Antal

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Electronic, nanopore based single molecule real-time DNA sequencing technology offers very long, albeit lower accuracy reads in sharp contrast to existing next-generation sequencing methods, which offer short, high-accuracy reads in abundance.We provide a systematic review of the error characteristics of this new sequencing platform, and demonstrate the most challenging aspects in the field of whole gene sequencing through the human HLA-DQA2 gene using long-range PCR products on multiplexed samples. We consider the limitations of these errors for the applications of this technology, and also indicate prospective improvements and expected thresholds with respect to these errors.

Original languageEnglish
Pages (from-to)231-237
Number of pages7
JournalPeriodica polytechnica Electrical engineering and computer science
Volume61
Issue number3
DOIs
Publication statusPublished - Aug 17 2017

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Keywords

  • DNA sequencing
  • Error characteristics
  • Nanopore
  • Next-generation sequencing

ASJC Scopus subject areas

  • Software
  • Signal Processing
  • Information Systems
  • Computer Science Applications
  • Computer Networks and Communications
  • Electrical and Electronic Engineering

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