Betulinic acid in complex with a gamma-cyclodextrin derivative decreases proliferation and in vivo tumor development of non-metastatic and metastatic B164A5 cells

Codruta Soica, Corina Danciu, Germaine Savoiu-Balint, Florin Borcan, Rita Ambrus, Istvan Zupko, Florina Bojin, Dorina Coricovac, Sorina Ciurlea, Stefana Avram, Cristina Adriana Dehelean, Teodora Olariu, Petru Matusz

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48 Citations (Scopus)

Abstract

Betulinic acid, a very promising anti-melanoma agent, has very low water solubility that causes low bioavailability. To overcome this inconvenience, a highly water-soluble cyclodextrin was used (octakis-[6-deoxy-6-(2-sulfanyl ethanesulfonic acid)]-γ-cyclodextrin). The complex was physico-chemically analyzed using differential scanning calorimetry (DSC), X-ray and scanning electron microscopy (SEM) methods and then in vitro tested for its antiproliferative activity by the MTT assay and by cell cycle analysis. Finally, the complex was tested in vivo using an animal model of murine melanoma developed in C57BL/6J mice, where it caused a reduction in tumor volume and weight. The study revealed the beneficial influence of betulinic acid inclusion into the cyclodextrin in terms of antiproliferative activity and in vivo tumor development.

Original languageEnglish
Pages (from-to)8235-8255
Number of pages21
JournalInternational journal of molecular sciences
Volume15
Issue number5
DOIs
Publication statusPublished - May 9 2014

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Keywords

  • Betulinic acid
  • C57BL/6J mice
  • DSC
  • MTT test
  • SEM
  • X-ray diffraction

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Soica, C., Danciu, C., Savoiu-Balint, G., Borcan, F., Ambrus, R., Zupko, I., Bojin, F., Coricovac, D., Ciurlea, S., Avram, S., Dehelean, C. A., Olariu, T., & Matusz, P. (2014). Betulinic acid in complex with a gamma-cyclodextrin derivative decreases proliferation and in vivo tumor development of non-metastatic and metastatic B164A5 cells. International journal of molecular sciences, 15(5), 8235-8255. https://doi.org/10.3390/ijms15058235