Beta-adrenergic receptors in early human placenta characterization of [3H]-dihydroalprenolol binding

G. Falkay, L. Kovacs

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The binding characteristics of beta-adrenergic ligand [3H]-dihydroalprenolol (DHA) were determined in particulate membranes of early human placenta (8 - 12 weeks of gestation). [3H]-DHA binding to crude membrane fractions was rapid, reversible, saturable and linearly correlated with the membrane protein concentration. Scatchard analysis of saturation experiments showed a KD of 2.80 ± 0.9 nM and a density of binding sites of 330.30 ± 93.5 fmol/mg protein. Agonist potency isoproterenol epinephrine norepinephrine indicated that early human placenta contains an adrenergic receptor of beta-2 subtype.

Original languageEnglish
Pages (from-to)1583-1590
Number of pages8
JournalLife Sciences
Volume32
Issue number14
DOIs
Publication statusPublished - Apr 4 1983

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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