Best practice of identification and proteomic analysis of extracellular vesicles in human health and disease

Barbara W. Sódar, Árpád Kovács, Tamás Visnovitz, E. Pállinger, K. Vékey, Gabriella Pocsfalvi, Lilla Turiák, E. Búzás

Research output: Contribution to journalReview article

12 Citations (Scopus)

Abstract

Introduction: Extracellular vesicles are emerging sources of biomarkers for modern preventive and precision medicine. Extracellular vesicles in body fluids offer a unique opportunity for integrative biomarker approaches due to their complex biocargo that includes proteins, lipids, nucleic acids and metabolites. Mass spectrometry-based proteomics data suggest that a significant portion of human proteins are sorted into extracellular vesicles and amenable for biomarker discovery schemes. Areas covered: this review focuses on key aspects of isolation, quality control and subsequent analysis of blood plasma- and conditioned medium-derived extracellular vesicle proteins, and summarizes the current state-of-the-art in the field. Furthermore, it provides introduction and guidelines for mass spectrometry-based proteomic analysis of extracellular vesicles. Expert commentary: Comparison of newly developed isolation and purification techniques with classical ultracentrifugation-based approaches are highly recommended. It is also essential to use multiple analytical approaches to characterize the isolated extracellular vesicles prior to characterization of their biocargo. Rigor in data reproducibility, critical data analysis, awareness of potential pitfalls, standardization and benchmarking are required for extracellular vesicle research to fulfil the current expectation that these subcellular structures can become a valid source of next generation biomarkers.

Original languageEnglish
Pages (from-to)1073-1090
Number of pages18
JournalExpert Review of Proteomics
Volume14
Issue number12
DOIs
Publication statusPublished - Dec 2 2017

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Biomarkers
Practice Guidelines
Proteomics
Health
Mass spectrometry
Proteins
Body fluids
Benchmarking
Conditioned Culture Medium
Metabolites
Mass Spectrometry
Standardization
Nucleic Acids
Medicine
Purification
Quality control
Blood
Precision Medicine
Modern 1601-history
Preventive Medicine

Keywords

  • Exosomes
  • extracellular vesicles
  • mass spectrometry
  • microvesicles
  • proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Best practice of identification and proteomic analysis of extracellular vesicles in human health and disease. / Sódar, Barbara W.; Kovács, Árpád; Visnovitz, Tamás; Pállinger, E.; Vékey, K.; Pocsfalvi, Gabriella; Turiák, Lilla; Búzás, E.

In: Expert Review of Proteomics, Vol. 14, No. 12, 02.12.2017, p. 1073-1090.

Research output: Contribution to journalReview article

Sódar, Barbara W. ; Kovács, Árpád ; Visnovitz, Tamás ; Pállinger, E. ; Vékey, K. ; Pocsfalvi, Gabriella ; Turiák, Lilla ; Búzás, E. / Best practice of identification and proteomic analysis of extracellular vesicles in human health and disease. In: Expert Review of Proteomics. 2017 ; Vol. 14, No. 12. pp. 1073-1090.
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