Benzimidazole resistance in Haemonchus contortus recovered from farmed red deer

Gábor Nagy, Ágnes Csivincsik, A. Zsolnai, László Sugár

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Thirty Haemonchus contortus male worms were collected from farmed red deer yearlings in order to determine whether routine administration of albendazole for a long-term period (17 years) could select anthelmintic resistance. PCR–RFLP method based on single-nucleotide polymorphism of codon 200 in isotype 1 ß-tubulin gene (Phe200Tyr) was applied. The results showed a significant frequency of either the resistant allele (85 %) or the homozygous resistant genotype (70 %). By chi-square test, Hardy–Weinberg equilibrium of the population was accepted (p = 0.334, power of test 0.01). True prevalence of the resistant genotype (RR) was estimated to be 46.5–87.2 % (confidence interval 95 %) calculated by Sterne’s exact method. These results confirmed that long-term use of benzimidazoles could change the relative allele frequency of genes associated with drug resistance and may cause a large-scale spread of the resistant allele. To our knowledge, this study supported benzimidazole resistance in Haemonchus contortus in red deer for the first time.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalParasitology Research
DOIs
Publication statusAccepted/In press - Jun 1 2016

Fingerprint

Haemonchus
benzimidazole
Deer
Haemonchus contortus
Cervus elaphus
Alleles
Genotype
Benzimidazoles
benzimidazoles
alleles
Albendazole
albendazole
Anthelmintics
genotype
drug resistance
Chi-Square Distribution
Tubulin
tubulin
anthelmintics
Drug Resistance

Keywords

  • Albendazole
  • Haemonchus contortus
  • Red deer
  • Resistance

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Insect Science
  • veterinary(all)

Cite this

Benzimidazole resistance in Haemonchus contortus recovered from farmed red deer. / Nagy, Gábor; Csivincsik, Ágnes; Zsolnai, A.; Sugár, László.

In: Parasitology Research, 01.06.2016, p. 1-5.

Research output: Contribution to journalArticle

Nagy, Gábor ; Csivincsik, Ágnes ; Zsolnai, A. ; Sugár, László. / Benzimidazole resistance in Haemonchus contortus recovered from farmed red deer. In: Parasitology Research. 2016 ; pp. 1-5.
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