Benzamil prevents brain water accumulation in hyponatraemic rats

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: It has been recently shown that A6 cells exposed to hyponatraemic stress respond with increased sodium uptake via activation of benzamil-sensitive sodium channels. This study was performed, therefore, to explore the possible involvement of benzamil-sensitive sodium channels and cellular sodium influx in brain oedema formation in hyponatraemic rats. Methods: Four groups of male Wistar rats were studied (n∈=∈13 in each group). Animals in group I with normonatraemia received intracerebroventricular (icv) 0.9% NaCl; animals in group II-IV were made hyponatraemic by intraperitoneal administration of isotonic glucose solution in a dose of 20% per body weight. Rats were pretreated with icv 0.9% NaCl (group II), 120 μg arginine vasopressin (AVP) (group III) or 4 μg benzamil-hydrochloride (group IV). Plasma sodium (ion-selective electrode) plasma osmolality (vapour pressure osmometer) and brain sodium and potassium content (flame photometer) as well as brain water content (desiccation method) were measured after a 2-h hydration period. Results: Plasma sodium, osmolality and tissue sodium and potassium contents were markedly depressed in hyponatraemic rats (group II-IV, p∈

Original languageEnglish
Pages (from-to)1121-1125
Number of pages5
JournalActa Neurochirurgica
Volume151
Issue number9
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Sodium
Water
Brain
Sodium Channels
Osmolar Concentration
Potassium
Isotonic Solutions
Ion-Selective Electrodes
Vapor Pressure
Desiccation
Arginine Vasopressin
Brain Edema
benzamil
Wistar Rats
Body Weight
Glucose

Keywords

  • Benzamil
  • Brain oedema
  • Hyponatraemia
  • Sodium channel

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Benzamil prevents brain water accumulation in hyponatraemic rats. / Sulyok, E.; Pál, J.; Vajda, Z.; Steier, Roy; Dóczi, T.

In: Acta Neurochirurgica, Vol. 151, No. 9, 09.2009, p. 1121-1125.

Research output: Contribution to journalArticle

@article{1f8c8f30ea9a4835bfc6392c481b74a7,
title = "Benzamil prevents brain water accumulation in hyponatraemic rats",
abstract = "Background: It has been recently shown that A6 cells exposed to hyponatraemic stress respond with increased sodium uptake via activation of benzamil-sensitive sodium channels. This study was performed, therefore, to explore the possible involvement of benzamil-sensitive sodium channels and cellular sodium influx in brain oedema formation in hyponatraemic rats. Methods: Four groups of male Wistar rats were studied (n∈=∈13 in each group). Animals in group I with normonatraemia received intracerebroventricular (icv) 0.9{\%} NaCl; animals in group II-IV were made hyponatraemic by intraperitoneal administration of isotonic glucose solution in a dose of 20{\%} per body weight. Rats were pretreated with icv 0.9{\%} NaCl (group II), 120 μg arginine vasopressin (AVP) (group III) or 4 μg benzamil-hydrochloride (group IV). Plasma sodium (ion-selective electrode) plasma osmolality (vapour pressure osmometer) and brain sodium and potassium content (flame photometer) as well as brain water content (desiccation method) were measured after a 2-h hydration period. Results: Plasma sodium, osmolality and tissue sodium and potassium contents were markedly depressed in hyponatraemic rats (group II-IV, p∈",
keywords = "Benzamil, Brain oedema, Hyponatraemia, Sodium channel",
author = "E. Sulyok and J. P{\'a}l and Z. Vajda and Roy Steier and T. D{\'o}czi",
year = "2009",
month = "9",
doi = "10.1007/s00701-009-0354-x",
language = "English",
volume = "151",
pages = "1121--1125",
journal = "Acta Neurochirurgica",
issn = "0001-6268",
publisher = "Springer Wien",
number = "9",

}

TY - JOUR

T1 - Benzamil prevents brain water accumulation in hyponatraemic rats

AU - Sulyok, E.

AU - Pál, J.

AU - Vajda, Z.

AU - Steier, Roy

AU - Dóczi, T.

PY - 2009/9

Y1 - 2009/9

N2 - Background: It has been recently shown that A6 cells exposed to hyponatraemic stress respond with increased sodium uptake via activation of benzamil-sensitive sodium channels. This study was performed, therefore, to explore the possible involvement of benzamil-sensitive sodium channels and cellular sodium influx in brain oedema formation in hyponatraemic rats. Methods: Four groups of male Wistar rats were studied (n∈=∈13 in each group). Animals in group I with normonatraemia received intracerebroventricular (icv) 0.9% NaCl; animals in group II-IV were made hyponatraemic by intraperitoneal administration of isotonic glucose solution in a dose of 20% per body weight. Rats were pretreated with icv 0.9% NaCl (group II), 120 μg arginine vasopressin (AVP) (group III) or 4 μg benzamil-hydrochloride (group IV). Plasma sodium (ion-selective electrode) plasma osmolality (vapour pressure osmometer) and brain sodium and potassium content (flame photometer) as well as brain water content (desiccation method) were measured after a 2-h hydration period. Results: Plasma sodium, osmolality and tissue sodium and potassium contents were markedly depressed in hyponatraemic rats (group II-IV, p∈

AB - Background: It has been recently shown that A6 cells exposed to hyponatraemic stress respond with increased sodium uptake via activation of benzamil-sensitive sodium channels. This study was performed, therefore, to explore the possible involvement of benzamil-sensitive sodium channels and cellular sodium influx in brain oedema formation in hyponatraemic rats. Methods: Four groups of male Wistar rats were studied (n∈=∈13 in each group). Animals in group I with normonatraemia received intracerebroventricular (icv) 0.9% NaCl; animals in group II-IV were made hyponatraemic by intraperitoneal administration of isotonic glucose solution in a dose of 20% per body weight. Rats were pretreated with icv 0.9% NaCl (group II), 120 μg arginine vasopressin (AVP) (group III) or 4 μg benzamil-hydrochloride (group IV). Plasma sodium (ion-selective electrode) plasma osmolality (vapour pressure osmometer) and brain sodium and potassium content (flame photometer) as well as brain water content (desiccation method) were measured after a 2-h hydration period. Results: Plasma sodium, osmolality and tissue sodium and potassium contents were markedly depressed in hyponatraemic rats (group II-IV, p∈

KW - Benzamil

KW - Brain oedema

KW - Hyponatraemia

KW - Sodium channel

UR - http://www.scopus.com/inward/record.url?scp=69249211262&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69249211262&partnerID=8YFLogxK

U2 - 10.1007/s00701-009-0354-x

DO - 10.1007/s00701-009-0354-x

M3 - Article

VL - 151

SP - 1121

EP - 1125

JO - Acta Neurochirurgica

JF - Acta Neurochirurgica

SN - 0001-6268

IS - 9

ER -