Beneficial Effects of Phosphatidylcholine During Hindlimb Reperfusion

László Gera, Renáta Varga, László Török, J. Kaszaki, Andrea Szabó, K. Nagy, M. Borós

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Microcirculatory dysfunctions and mast cell (MC) reactions play important roles in hypoxic tissue injuries. The aims of this study were to characterize the effects of hindlimb ischemia-reperfusion (I-R) on the periosteal microcirculation and to define the consequences of systemic phosphatidylcholine (PC) therapy during this condition. Materials and methods: Microcirculatory changes were visualized by means of fluorescence intravital videomicroscopy in anesthetized Wistar rats. There was 60 min of complete hindlimb ischemia followed by a 180-min reperfusion in the presence of PC treatment (50 mg/kg i.v.; in the second 10 min of reperfusion) or vehicle. Further two groups served as vehicle- or PC-treated sham-operated controls. The proportion of degranulated MCs and the leukocyte accumulation (myeloperoxidase, MPO assay) were determined in muscle biopsies. Results: I-R significantly increased the muscle MPO activity (from 14.94 to 63.45 mU/mg) and the proportion of degranulated MCs (to 82.5%). The periosteal capillary RBC velocity (RBCV) and the functional capillary density (FCD) had decreased, while the primary and secondary leukocyte-endothelial cell interactions had increased by the end of reperfusion (rolling from 20.8 to 40.0%, and firm adherence from 254 to 872 mm-2). PC treatment decreased the leukocyte rolling and sticking, preserved the FCD and improved the RBCV. The MC degranulation and MPO activity diminished significantly in the muscle layer. Conclusions: PC administration improves I-R-induced periosteal microcirculatory dysfunctions and ameliorates secondary inflammatory reactions. Systemic PC treatment could offer a potential treatment modality during hypoperfusion or inflammatory conditions of the bones.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalJournal of Surgical Research
Volume139
Issue number1
DOIs
Publication statusPublished - May 1 2007

Fingerprint

Hindlimb
Phosphatidylcholines
Reperfusion
Ischemia
Mast Cells
Muscles
Leukocytes
Leukocyte Rolling
Therapeutics
Cell Degranulation
Video Microscopy
Microcirculation
Cell Communication
Peroxidase
Wistar Rats
Endothelial Cells
Fluorescence
Biopsy
Bone and Bones
Wounds and Injuries

Keywords

  • leukocyte
  • mast cell
  • periosteum
  • rat
  • videomicroscopy

ASJC Scopus subject areas

  • Surgery

Cite this

Beneficial Effects of Phosphatidylcholine During Hindlimb Reperfusion. / Gera, László; Varga, Renáta; Török, László; Kaszaki, J.; Szabó, Andrea; Nagy, K.; Borós, M.

In: Journal of Surgical Research, Vol. 139, No. 1, 01.05.2007, p. 45-50.

Research output: Contribution to journalArticle

Gera, László ; Varga, Renáta ; Török, László ; Kaszaki, J. ; Szabó, Andrea ; Nagy, K. ; Borós, M. / Beneficial Effects of Phosphatidylcholine During Hindlimb Reperfusion. In: Journal of Surgical Research. 2007 ; Vol. 139, No. 1. pp. 45-50.
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AU - Gera, László

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AU - Török, László

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AU - Szabó, Andrea

AU - Nagy, K.

AU - Borós, M.

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AB - Background: Microcirculatory dysfunctions and mast cell (MC) reactions play important roles in hypoxic tissue injuries. The aims of this study were to characterize the effects of hindlimb ischemia-reperfusion (I-R) on the periosteal microcirculation and to define the consequences of systemic phosphatidylcholine (PC) therapy during this condition. Materials and methods: Microcirculatory changes were visualized by means of fluorescence intravital videomicroscopy in anesthetized Wistar rats. There was 60 min of complete hindlimb ischemia followed by a 180-min reperfusion in the presence of PC treatment (50 mg/kg i.v.; in the second 10 min of reperfusion) or vehicle. Further two groups served as vehicle- or PC-treated sham-operated controls. The proportion of degranulated MCs and the leukocyte accumulation (myeloperoxidase, MPO assay) were determined in muscle biopsies. Results: I-R significantly increased the muscle MPO activity (from 14.94 to 63.45 mU/mg) and the proportion of degranulated MCs (to 82.5%). The periosteal capillary RBC velocity (RBCV) and the functional capillary density (FCD) had decreased, while the primary and secondary leukocyte-endothelial cell interactions had increased by the end of reperfusion (rolling from 20.8 to 40.0%, and firm adherence from 254 to 872 mm-2). PC treatment decreased the leukocyte rolling and sticking, preserved the FCD and improved the RBCV. The MC degranulation and MPO activity diminished significantly in the muscle layer. Conclusions: PC administration improves I-R-induced periosteal microcirculatory dysfunctions and ameliorates secondary inflammatory reactions. Systemic PC treatment could offer a potential treatment modality during hypoperfusion or inflammatory conditions of the bones.

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