Beneficial effects of pentoxifylline treatment of experimental acute pancreatitis in rats

J. Marton, G. Farkas, T. Takács, Z. Nagy, Z. Szasz, J. Varga, K. Jármay, A. Balogh, J. Lonovics

Research output: Contribution to journalArticle

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Abstract

The purposes of this study were to determine the tumor necrosis factor (TNF) and interleukin-6 (IL-6) levels after the induction of acute necrotizing pancreatitis, and to establish the effects of pentoxifylline on cytokine production. Methods: acute pancreatitis was induced by the retrograde injection of 200 μl taurochulic acid into the pancreatic duct in male Wistar rats. The serum amylase activity, the wet pancreatic weight/body weight ratio, and the TNF and IL-6 levels were measured. Seven mg/kg pentoxifylline were administered intraperitoneally at the rime of operation 6, 12 or 24 h later. Rats were killed 6, 24, 48 or 72 h after the operation. Results: the TNF bioassay revealed high levels of TNF (30.2 ± 5.4 U/ml, 35.0 ± 5.0 U/ml and 36.6 ± 6.0 U/ml) in the control group at 6, 24 and 48 h and (54.1 ± 20 U/ml and 10.9 ± 4.2 U/ml) in the pentoxifylline-treated group at 6 and 24 h, respectively, whereas the level had decreased to zero in the pentoxifylline-treated group at 48 h. The IL-6 bioassay likewise demonstrated high levels of IL-6 in the control group at 48 h and in the pentoxifylline-treated group at 6 and 24 h, and markedly decreased levels in the pentoxifylline-treated group at 48 h (7083 ± 2844 pg/ml, 6463 ± 1307 pg/ml, 10329 ± 5571 pg/ml vs 137.5 ± 85.5 pg/ml, respectively, P <0.05). The high mortality observed in the pancreatitis group (43%) was decreased by pentoxifylline administration to 11%. Conclusion: these results demonstrate that pentoxifylline very effectively inhibits cytokine production in acute pancreatitis.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalResearch in Experimental Medicine
Volume197
Issue number5
DOIs
Publication statusPublished - 1998

Fingerprint

Pentoxifylline
Pancreatitis
Interleukin-6
Tumor Necrosis Factor-alpha
Therapeutics
Biological Assay
Acute Necrotizing Pancreatitis
Cytokines
Control Groups
Pancreatic Ducts
Amylases
Wistar Rats
Body Weight
Weights and Measures
Injections
Acids
Mortality

Keywords

  • Experimental acute pancreatitis
  • Interleukin-6
  • Pentoxifylline
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Beneficial effects of pentoxifylline treatment of experimental acute pancreatitis in rats. / Marton, J.; Farkas, G.; Takács, T.; Nagy, Z.; Szasz, Z.; Varga, J.; Jármay, K.; Balogh, A.; Lonovics, J.

In: Research in Experimental Medicine, Vol. 197, No. 5, 1998, p. 293-299.

Research output: Contribution to journalArticle

Marton, J. ; Farkas, G. ; Takács, T. ; Nagy, Z. ; Szasz, Z. ; Varga, J. ; Jármay, K. ; Balogh, A. ; Lonovics, J. / Beneficial effects of pentoxifylline treatment of experimental acute pancreatitis in rats. In: Research in Experimental Medicine. 1998 ; Vol. 197, No. 5. pp. 293-299.
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abstract = "The purposes of this study were to determine the tumor necrosis factor (TNF) and interleukin-6 (IL-6) levels after the induction of acute necrotizing pancreatitis, and to establish the effects of pentoxifylline on cytokine production. Methods: acute pancreatitis was induced by the retrograde injection of 200 μl taurochulic acid into the pancreatic duct in male Wistar rats. The serum amylase activity, the wet pancreatic weight/body weight ratio, and the TNF and IL-6 levels were measured. Seven mg/kg pentoxifylline were administered intraperitoneally at the rime of operation 6, 12 or 24 h later. Rats were killed 6, 24, 48 or 72 h after the operation. Results: the TNF bioassay revealed high levels of TNF (30.2 ± 5.4 U/ml, 35.0 ± 5.0 U/ml and 36.6 ± 6.0 U/ml) in the control group at 6, 24 and 48 h and (54.1 ± 20 U/ml and 10.9 ± 4.2 U/ml) in the pentoxifylline-treated group at 6 and 24 h, respectively, whereas the level had decreased to zero in the pentoxifylline-treated group at 48 h. The IL-6 bioassay likewise demonstrated high levels of IL-6 in the control group at 48 h and in the pentoxifylline-treated group at 6 and 24 h, and markedly decreased levels in the pentoxifylline-treated group at 48 h (7083 ± 2844 pg/ml, 6463 ± 1307 pg/ml, 10329 ± 5571 pg/ml vs 137.5 ± 85.5 pg/ml, respectively, P <0.05). The high mortality observed in the pancreatitis group (43{\%}) was decreased by pentoxifylline administration to 11{\%}. Conclusion: these results demonstrate that pentoxifylline very effectively inhibits cytokine production in acute pancreatitis.",
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AU - Szasz, Z.

AU - Varga, J.

AU - Jármay, K.

AU - Balogh, A.

AU - Lonovics, J.

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N2 - The purposes of this study were to determine the tumor necrosis factor (TNF) and interleukin-6 (IL-6) levels after the induction of acute necrotizing pancreatitis, and to establish the effects of pentoxifylline on cytokine production. Methods: acute pancreatitis was induced by the retrograde injection of 200 μl taurochulic acid into the pancreatic duct in male Wistar rats. The serum amylase activity, the wet pancreatic weight/body weight ratio, and the TNF and IL-6 levels were measured. Seven mg/kg pentoxifylline were administered intraperitoneally at the rime of operation 6, 12 or 24 h later. Rats were killed 6, 24, 48 or 72 h after the operation. Results: the TNF bioassay revealed high levels of TNF (30.2 ± 5.4 U/ml, 35.0 ± 5.0 U/ml and 36.6 ± 6.0 U/ml) in the control group at 6, 24 and 48 h and (54.1 ± 20 U/ml and 10.9 ± 4.2 U/ml) in the pentoxifylline-treated group at 6 and 24 h, respectively, whereas the level had decreased to zero in the pentoxifylline-treated group at 48 h. The IL-6 bioassay likewise demonstrated high levels of IL-6 in the control group at 48 h and in the pentoxifylline-treated group at 6 and 24 h, and markedly decreased levels in the pentoxifylline-treated group at 48 h (7083 ± 2844 pg/ml, 6463 ± 1307 pg/ml, 10329 ± 5571 pg/ml vs 137.5 ± 85.5 pg/ml, respectively, P <0.05). The high mortality observed in the pancreatitis group (43%) was decreased by pentoxifylline administration to 11%. Conclusion: these results demonstrate that pentoxifylline very effectively inhibits cytokine production in acute pancreatitis.

AB - The purposes of this study were to determine the tumor necrosis factor (TNF) and interleukin-6 (IL-6) levels after the induction of acute necrotizing pancreatitis, and to establish the effects of pentoxifylline on cytokine production. Methods: acute pancreatitis was induced by the retrograde injection of 200 μl taurochulic acid into the pancreatic duct in male Wistar rats. The serum amylase activity, the wet pancreatic weight/body weight ratio, and the TNF and IL-6 levels were measured. Seven mg/kg pentoxifylline were administered intraperitoneally at the rime of operation 6, 12 or 24 h later. Rats were killed 6, 24, 48 or 72 h after the operation. Results: the TNF bioassay revealed high levels of TNF (30.2 ± 5.4 U/ml, 35.0 ± 5.0 U/ml and 36.6 ± 6.0 U/ml) in the control group at 6, 24 and 48 h and (54.1 ± 20 U/ml and 10.9 ± 4.2 U/ml) in the pentoxifylline-treated group at 6 and 24 h, respectively, whereas the level had decreased to zero in the pentoxifylline-treated group at 48 h. The IL-6 bioassay likewise demonstrated high levels of IL-6 in the control group at 48 h and in the pentoxifylline-treated group at 6 and 24 h, and markedly decreased levels in the pentoxifylline-treated group at 48 h (7083 ± 2844 pg/ml, 6463 ± 1307 pg/ml, 10329 ± 5571 pg/ml vs 137.5 ± 85.5 pg/ml, respectively, P <0.05). The high mortality observed in the pancreatitis group (43%) was decreased by pentoxifylline administration to 11%. Conclusion: these results demonstrate that pentoxifylline very effectively inhibits cytokine production in acute pancreatitis.

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