Beneficial effect of octreotide treatment in acute pancreatitis in rats

Janos Marton, Zsuzsanna Szasz, Zsuzsanna Nagy, K. Jármay, T. Takács, J. Lonovics, Adam Balogh, Gyula Farkas

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Conclusions: Octreotide treatment contributes to the regulation of tumor necrosis factor (TNF) production in sodium taurocholate-induced acute necrotizing pancreatitis in rats. Owing to its complex effect, octreotide can partially ameliorate the deleterious consequences of acute necrotizing pancreatitis. Elevated TNF and interleukin-6 (IL-6) levels in the peritoneal fluid may be considered a consequence of the activation of peritoneal macrophages. Background. The effects of octreotide on exocrine pancreatic function have been investigated in numerous studies, but little attention has been paid to its influence on cytokine production in acute pancreatitis. Methods. Acute pancreatitis was induced by the retrograde injection of taurocholic acid into the pancreatic duct in male Wistar rats. Serum amylase activity, wet pancreatic weight/body weight (pw/bw) ratio, and TNF and IL-6 levels were measured. Four μg/kg of octreotide was administered subcutaneously at the time of induction of pancreatitis and 24 or 48 h later. Rats were sacrificed 6, 24, 48, or 72 h after the operation. Results. The serum amylase level and pancreatic weight to body weight ratio were decreased significantly in the octreotide-treated group. The serum TNF level was decreased significantly in the octreotide-treated group as compared with the control group at 6, 24, and 48 h (0.6 ± 1.5, 2.0 ± 3.3, and 0 vs 50 ± 15.5, 37.5 ± 18.4, and 13.1 ± 12.5 U/mL, respectively). The ascites TNF level was decreased to 0 in the octreotide-treated group and was elevated in the control group at 72 h (28.0 ± 49.0 U/mL). IL-6 production in ascites was extremely high in both groups at 6 h (80,000 ± 43, 817 pg/mL and 58, 500 ± 33 335 pg/mL), but the difference was not significant.

Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalInternational Journal of Pancreatology
Volume24
Issue number3
Publication statusPublished - 1998

Fingerprint

Octreotide
Pancreatitis
Tumor Necrosis Factor-alpha
Acute Necrotizing Pancreatitis
Interleukin-6
Taurocholic Acid
Amylases
Ascites
Therapeutics
Serum
Body Weight
Weights and Measures
Control Groups
Pancreatic Ducts
Ascitic Fluid
Peritoneal Macrophages
Wistar Rats
Cytokines
Injections

Keywords

  • Acute pancreatitis
  • IL-6
  • Octreotide
  • TNF
  • TNF-α

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology
  • Oncology

Cite this

Beneficial effect of octreotide treatment in acute pancreatitis in rats. / Marton, Janos; Szasz, Zsuzsanna; Nagy, Zsuzsanna; Jármay, K.; Takács, T.; Lonovics, J.; Balogh, Adam; Farkas, Gyula.

In: International Journal of Pancreatology, Vol. 24, No. 3, 1998, p. 203-210.

Research output: Contribution to journalArticle

Marton, Janos ; Szasz, Zsuzsanna ; Nagy, Zsuzsanna ; Jármay, K. ; Takács, T. ; Lonovics, J. ; Balogh, Adam ; Farkas, Gyula. / Beneficial effect of octreotide treatment in acute pancreatitis in rats. In: International Journal of Pancreatology. 1998 ; Vol. 24, No. 3. pp. 203-210.
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AU - Marton, Janos

AU - Szasz, Zsuzsanna

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AU - Takács, T.

AU - Lonovics, J.

AU - Balogh, Adam

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N2 - Conclusions: Octreotide treatment contributes to the regulation of tumor necrosis factor (TNF) production in sodium taurocholate-induced acute necrotizing pancreatitis in rats. Owing to its complex effect, octreotide can partially ameliorate the deleterious consequences of acute necrotizing pancreatitis. Elevated TNF and interleukin-6 (IL-6) levels in the peritoneal fluid may be considered a consequence of the activation of peritoneal macrophages. Background. The effects of octreotide on exocrine pancreatic function have been investigated in numerous studies, but little attention has been paid to its influence on cytokine production in acute pancreatitis. Methods. Acute pancreatitis was induced by the retrograde injection of taurocholic acid into the pancreatic duct in male Wistar rats. Serum amylase activity, wet pancreatic weight/body weight (pw/bw) ratio, and TNF and IL-6 levels were measured. Four μg/kg of octreotide was administered subcutaneously at the time of induction of pancreatitis and 24 or 48 h later. Rats were sacrificed 6, 24, 48, or 72 h after the operation. Results. The serum amylase level and pancreatic weight to body weight ratio were decreased significantly in the octreotide-treated group. The serum TNF level was decreased significantly in the octreotide-treated group as compared with the control group at 6, 24, and 48 h (0.6 ± 1.5, 2.0 ± 3.3, and 0 vs 50 ± 15.5, 37.5 ± 18.4, and 13.1 ± 12.5 U/mL, respectively). The ascites TNF level was decreased to 0 in the octreotide-treated group and was elevated in the control group at 72 h (28.0 ± 49.0 U/mL). IL-6 production in ascites was extremely high in both groups at 6 h (80,000 ± 43, 817 pg/mL and 58, 500 ± 33 335 pg/mL), but the difference was not significant.

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