Beneficial effect of a human monoclonal IgM cryoglobulin on the autoimmune disease of New Zealand black mice

F. Uher, Puskás Éva Puskás, J. Cervenak

Research output: Contribution to journalArticle

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Abstract

NZB mice spontaneously develop an autoimmune disease characterized by autoimmune hemolytic anemia, thymic atrophy, lymphoid hyperplasia, and hypergammaglobulinemia. The aim of this study was to examine the hypothesis that cryoglobulins may have an immunoregulatory effect on the autoimmune process. The effect of human monoclonal IgM cryoglobulin preparations (including Cryo13, Cryo14, and Cryo16) isolated from the serum of patients with Waldenström's macroglobulinemia on the autoimmune disease of NZB mice was therefore studied. The effect of cryoglobulin preparations was evaluated on several disease parameters,i.e., survival, severity of anemia, and serum IgM and IgG levels (hypergammaglobulinemia). We found that immunization of NZB mice with Cryo13 at 3 months of age delayed the course of the disease, whereas Cryo14 and Cryo16 were ineffective. Furthermore, the effect of Cryo13 was long lasting. On the other hand, Cryo13 was able to react with 8 of 32 mouse monoclonal natural IgM autoantibodies. In contrast, Cryo14 was able to bind only 2 and Cryo16 none of these mouse monoclonal IgM antibodies. These results indicate that, in this model of autoimmune pathology, the beneficial effect of Cryo13 is mediated by its idiotypic interaction with the murine natural auto-antibody network.

Original languageEnglish
Pages (from-to)136-141
Number of pages6
JournalCellular Immunology
Volume206
Issue number2
DOIs
Publication statusPublished - Dec 15 2000

Fingerprint

Cryoglobulins
Inbred NZB Mouse
New Zealand
Autoimmune Diseases
Immunoglobulin M
Hypergammaglobulinemia
Waldenstrom Macroglobulinemia
Autoimmune Hemolytic Anemia
Serum
Autoantibodies
Atrophy
Hyperplasia
Anemia
Immunization
Immunoglobulin G
Monoclonal Antibodies
Pathology
Survival
Antibodies

Keywords

  • Cryoglobulins
  • Idiotypic network
  • Lupus
  • Natural autoantibodies
  • NZB mice
  • Rheumatoid factors

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Beneficial effect of a human monoclonal IgM cryoglobulin on the autoimmune disease of New Zealand black mice. / Uher, F.; Éva Puskás, Puskás; Cervenak, J.

In: Cellular Immunology, Vol. 206, No. 2, 15.12.2000, p. 136-141.

Research output: Contribution to journalArticle

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