Bead arrays for antibody and complement profiling reveal joint contribution of antibody isotypes to C3 deposition

Burcu Ayoglu, Eszter Szarka, Krisztina Huber, Anita Orosz, Fruzsina Babos, Anna Magyar, Ferenc Hudecz, Bernadette Rojkovich, Tamás Gáti, György Nagy, Jochen M. Schwenk, Gabriella Sármay, József Prechl, Peter Nilsson, Krisztián Papp

Research output: Contribution to journalArticle

9 Citations (Scopus)


The development of antigen arrays has provided researchers with great tools to identify reactivities against self or foreign antigens from body fluids. Yet, these approaches mostly do not address antibody isotypes and their effector functions even though these are key points for a more detailed understanding of disease processes. Here, we present a bead array-based assay for a multiplexed determination of antigen-specific antibody levels in parallel with their properties for complement activation. We measured the deposition of C3 fragments from serum samples to reflect the degree of complement activation via all three complement activation pathways. We utilized the assay on a bead array containing native and citrullinated peptide antigens to investigate the levels of IgG, IgM and IgA autoantibodies along with their complement activating properties in serum samples of 41 rheumatoid arthritis patients and 40 controls. Our analysis revealed significantly higher IgG reactivity against the citrullinated fibrinogen β and filaggrin peptides as well as an IgA reactivity that was exclusive for citrullinated fibrinogen β peptide and C3 deposition in rheumatoid arthritis patients. In addition, we characterized the humoral immune response against the viral EBNA-1 antigen to demonstrate the applicability of this assay beyond autoimmune conditions. We observed that particular buffer compositions were demanded for separate measurement of antibody reactivity and complement activation, as detection of antigen-antibody complexes appeared to be masked due to C3 deposition. We also found that rheumatoid factors of IgM isotype altered C3 deposition and introduced false-positive reactivities against EBNA-1 antigen. In conclusion, the presented bead-based assay setup can be utilized to profile antibody reactivities and immune-complex induced complement activation in a high-throughput manner and could facilitate the understanding and diagnosis of several diseases where complement activation plays role in the pathomechanism.

Original languageEnglish
Article numbere96403
JournalPloS one
Issue number5
Publication statusPublished - May 5 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Bead arrays for antibody and complement profiling reveal joint contribution of antibody isotypes to C3 deposition'. Together they form a unique fingerprint.

  • Cite this

    Ayoglu, B., Szarka, E., Huber, K., Orosz, A., Babos, F., Magyar, A., Hudecz, F., Rojkovich, B., Gáti, T., Nagy, G., Schwenk, J. M., Sármay, G., Prechl, J., Nilsson, P., & Papp, K. (2014). Bead arrays for antibody and complement profiling reveal joint contribution of antibody isotypes to C3 deposition. PloS one, 9(5), [e96403].