bcl-2 vs p53 Protein expression and apoptotic rate in human nonmelanoma skin cancers

N. Wikonkál, Rob J W Berg, Christian W. Van Haselen, Irene Horkay, E. Remenyik, Agnes Begany, J. Hunyadi, Willem A. Van Vloten, Frank R. De Gruijl

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Abstract

Background: A failure in the apoptotic response after severe genomic damage could facilitate cell transformation and tumor development, and a constitutive overexpression of either p53 or bcl-2 protein in nonapoptotic tumor cells could signify a defective bax-mediated apoptosis. Objectives: To investigate whether a negative correlation occurs between these 2 proteins in nonmelanoma skin cancer and whether overexpression of either protein is associated with a low rate of spontaneous apoptosis. Design: Immunohistochemical study of nonmelanoma skin cancer archive material. Setting: University referral center. Patients: White patients with tumors on sun-exposed skin areas (ie, 17 basal cell carcinomas and 22 squamous cell carcinomas). Main Outcome Measures: Positivity for p53 and bcl-2 were scored semiquantitatively on 4 levels, and the percentages of apoptotic cells were determined. Results: A significant negative correlation between p53 and bcl- 2 expression was found in the basal cell carcinomas, but not in the squamous cell carcinomas, largely attributable to the low level of bcl-2 staining in the squamous cell carcinomas. Squamous cell carcinomas have a significantly higher number of apoptotic cells than basal cell carcinomas: 1.1% vs 0.6%, respectively. This spontaneous apoptosis decreases with increasing bcl-2 (in basal cell carcinoma), whereas it does not appear to be related to p53 level expression. Conclusions: These results indicate that a disturbance in either p53 or bcl-2 suffices to enhance skin tumor formation by suppressing apoptosis; bcl-2 appears to reduce the rate of spontaneous apoptosis, but an aberrant p53 expression does not, and this factor may solely affect the apoptosis from exogenous genotoxicity.

Original languageEnglish
Pages (from-to)599-602
Number of pages4
JournalArchives of Dermatology
Volume133
Issue number5
Publication statusPublished - 1997

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Skin Neoplasms
Basal Cell Carcinoma
Apoptosis
Squamous Cell Carcinoma
Proteins
Neoplasms
Skin
Solar System
Referral and Consultation
Cell Count
Outcome Assessment (Health Care)
Staining and Labeling

ASJC Scopus subject areas

  • Dermatology

Cite this

Wikonkál, N., Berg, R. J. W., Van Haselen, C. W., Horkay, I., Remenyik, E., Begany, A., ... De Gruijl, F. R. (1997). bcl-2 vs p53 Protein expression and apoptotic rate in human nonmelanoma skin cancers. Archives of Dermatology, 133(5), 599-602.

bcl-2 vs p53 Protein expression and apoptotic rate in human nonmelanoma skin cancers. / Wikonkál, N.; Berg, Rob J W; Van Haselen, Christian W.; Horkay, Irene; Remenyik, E.; Begany, Agnes; Hunyadi, J.; Van Vloten, Willem A.; De Gruijl, Frank R.

In: Archives of Dermatology, Vol. 133, No. 5, 1997, p. 599-602.

Research output: Contribution to journalArticle

Wikonkál, N, Berg, RJW, Van Haselen, CW, Horkay, I, Remenyik, E, Begany, A, Hunyadi, J, Van Vloten, WA & De Gruijl, FR 1997, 'bcl-2 vs p53 Protein expression and apoptotic rate in human nonmelanoma skin cancers', Archives of Dermatology, vol. 133, no. 5, pp. 599-602.
Wikonkál, N. ; Berg, Rob J W ; Van Haselen, Christian W. ; Horkay, Irene ; Remenyik, E. ; Begany, Agnes ; Hunyadi, J. ; Van Vloten, Willem A. ; De Gruijl, Frank R. / bcl-2 vs p53 Protein expression and apoptotic rate in human nonmelanoma skin cancers. In: Archives of Dermatology. 1997 ; Vol. 133, No. 5. pp. 599-602.
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abstract = "Background: A failure in the apoptotic response after severe genomic damage could facilitate cell transformation and tumor development, and a constitutive overexpression of either p53 or bcl-2 protein in nonapoptotic tumor cells could signify a defective bax-mediated apoptosis. Objectives: To investigate whether a negative correlation occurs between these 2 proteins in nonmelanoma skin cancer and whether overexpression of either protein is associated with a low rate of spontaneous apoptosis. Design: Immunohistochemical study of nonmelanoma skin cancer archive material. Setting: University referral center. Patients: White patients with tumors on sun-exposed skin areas (ie, 17 basal cell carcinomas and 22 squamous cell carcinomas). Main Outcome Measures: Positivity for p53 and bcl-2 were scored semiquantitatively on 4 levels, and the percentages of apoptotic cells were determined. Results: A significant negative correlation between p53 and bcl- 2 expression was found in the basal cell carcinomas, but not in the squamous cell carcinomas, largely attributable to the low level of bcl-2 staining in the squamous cell carcinomas. Squamous cell carcinomas have a significantly higher number of apoptotic cells than basal cell carcinomas: 1.1{\%} vs 0.6{\%}, respectively. This spontaneous apoptosis decreases with increasing bcl-2 (in basal cell carcinoma), whereas it does not appear to be related to p53 level expression. Conclusions: These results indicate that a disturbance in either p53 or bcl-2 suffices to enhance skin tumor formation by suppressing apoptosis; bcl-2 appears to reduce the rate of spontaneous apoptosis, but an aberrant p53 expression does not, and this factor may solely affect the apoptosis from exogenous genotoxicity.",
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AU - Wikonkál, N.

AU - Berg, Rob J W

AU - Van Haselen, Christian W.

AU - Horkay, Irene

AU - Remenyik, E.

AU - Begany, Agnes

AU - Hunyadi, J.

AU - Van Vloten, Willem A.

AU - De Gruijl, Frank R.

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N2 - Background: A failure in the apoptotic response after severe genomic damage could facilitate cell transformation and tumor development, and a constitutive overexpression of either p53 or bcl-2 protein in nonapoptotic tumor cells could signify a defective bax-mediated apoptosis. Objectives: To investigate whether a negative correlation occurs between these 2 proteins in nonmelanoma skin cancer and whether overexpression of either protein is associated with a low rate of spontaneous apoptosis. Design: Immunohistochemical study of nonmelanoma skin cancer archive material. Setting: University referral center. Patients: White patients with tumors on sun-exposed skin areas (ie, 17 basal cell carcinomas and 22 squamous cell carcinomas). Main Outcome Measures: Positivity for p53 and bcl-2 were scored semiquantitatively on 4 levels, and the percentages of apoptotic cells were determined. Results: A significant negative correlation between p53 and bcl- 2 expression was found in the basal cell carcinomas, but not in the squamous cell carcinomas, largely attributable to the low level of bcl-2 staining in the squamous cell carcinomas. Squamous cell carcinomas have a significantly higher number of apoptotic cells than basal cell carcinomas: 1.1% vs 0.6%, respectively. This spontaneous apoptosis decreases with increasing bcl-2 (in basal cell carcinoma), whereas it does not appear to be related to p53 level expression. Conclusions: These results indicate that a disturbance in either p53 or bcl-2 suffices to enhance skin tumor formation by suppressing apoptosis; bcl-2 appears to reduce the rate of spontaneous apoptosis, but an aberrant p53 expression does not, and this factor may solely affect the apoptosis from exogenous genotoxicity.

AB - Background: A failure in the apoptotic response after severe genomic damage could facilitate cell transformation and tumor development, and a constitutive overexpression of either p53 or bcl-2 protein in nonapoptotic tumor cells could signify a defective bax-mediated apoptosis. Objectives: To investigate whether a negative correlation occurs between these 2 proteins in nonmelanoma skin cancer and whether overexpression of either protein is associated with a low rate of spontaneous apoptosis. Design: Immunohistochemical study of nonmelanoma skin cancer archive material. Setting: University referral center. Patients: White patients with tumors on sun-exposed skin areas (ie, 17 basal cell carcinomas and 22 squamous cell carcinomas). Main Outcome Measures: Positivity for p53 and bcl-2 were scored semiquantitatively on 4 levels, and the percentages of apoptotic cells were determined. Results: A significant negative correlation between p53 and bcl- 2 expression was found in the basal cell carcinomas, but not in the squamous cell carcinomas, largely attributable to the low level of bcl-2 staining in the squamous cell carcinomas. Squamous cell carcinomas have a significantly higher number of apoptotic cells than basal cell carcinomas: 1.1% vs 0.6%, respectively. This spontaneous apoptosis decreases with increasing bcl-2 (in basal cell carcinoma), whereas it does not appear to be related to p53 level expression. Conclusions: These results indicate that a disturbance in either p53 or bcl-2 suffices to enhance skin tumor formation by suppressing apoptosis; bcl-2 appears to reduce the rate of spontaneous apoptosis, but an aberrant p53 expression does not, and this factor may solely affect the apoptosis from exogenous genotoxicity.

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