Bcl-2 or Bcl-XL gene therapy reduces apoptosis and increases plasticity protein GAP-43 in PC12 cells

Aniko Gal, Geza Szilagyi, Edina Wappler, G. Sáfrány, Zoltan Nagy

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The anti-apoptotic gene replacement could be an option in preventing hypoxia induced neuronal loss-necrosis and/or apoptosis. This intervention is however still controversial. In this paper, we tested the bcl-2 or bcl-XL anti-apoptotic gene transfers using an adenovirus vector in PC12 cells after hypoxia and re-oxygenation. Gene delivery results in a significant increase in both Bcl-2 and Bcl-XL proteins expression. Hypoxia (1 h)/re-oxygenation (4-48 h) have a detrimental effect upon cultured cells by inducing increased apoptosis by 30% compared to the controls. After hypoxia the compromised mitochondrial membrane function was detected by decreased tetramethyl-rhodamine-ethylester (TMRE) staining. Anti-apoptotic genes transferred 1 h after hypoxia, prevent the cell damage; the number of apoptotic cells has been reduced significantly and the gene transfers prevent mitochondrial membrane damage. Under normoxic conditions or following hypoxia the expression of plasticity protein, growth associated protein 43 (GAP-43) increased significantly by the gene treatment. We can conclude that anti-apoptotic gene transfers are not only cytoprotective as it is already documented before but these genes activate GAP-43 as well. This link on apoptotic signals and cell plasticity is a new finding.

Original languageEnglish
Pages (from-to)349-353
Number of pages5
JournalBrain Research Bulletin
Volume76
Issue number4
DOIs
Publication statusPublished - Jul 1 2008

Fingerprint

GAP-43 Protein
PC12 Cells
Genetic Therapy
Apoptosis
Genes
Proteins
Cell Hypoxia
Mitochondrial Membranes
bcl-X Protein
Rhodamines
Adenoviridae
Cultured Cells
Necrosis
Cell Count
Staining and Labeling
Hypoxia

Keywords

  • Apoptosis
  • bcl-2 or bcl-XL gene transfer
  • GAP-43
  • Hypoxia
  • PC12

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bcl-2 or Bcl-XL gene therapy reduces apoptosis and increases plasticity protein GAP-43 in PC12 cells. / Gal, Aniko; Szilagyi, Geza; Wappler, Edina; Sáfrány, G.; Nagy, Zoltan.

In: Brain Research Bulletin, Vol. 76, No. 4, 01.07.2008, p. 349-353.

Research output: Contribution to journalArticle

Gal, Aniko ; Szilagyi, Geza ; Wappler, Edina ; Sáfrány, G. ; Nagy, Zoltan. / Bcl-2 or Bcl-XL gene therapy reduces apoptosis and increases plasticity protein GAP-43 in PC12 cells. In: Brain Research Bulletin. 2008 ; Vol. 76, No. 4. pp. 349-353.
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