Bcl-2 or bcl-XL gene therapy increases neural plasticity proteins nestin and c-fos expression in PC12 cells

Aniko Gal, Klara Pentelenyi, Viktoria Remenyi, Edina A. Wappler, G. Sáfrány, Judit Skopal, Zoltan Nagy

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The anti-apoptotic gene replacements could be an option in preventing hypoxia-induced neuronal loss. In this paper we tested the effect of anti-apoptosis (bcl-2 and bcl-XL) gene transfer on cell plasticity. Nestin, synapsin-1 and c-fos genes and proteins expression were measured in PC12 cells in normal condition, and after hypoxia/re-oxygenation. Gene delivery results a significant increase in both bcl-2 and bcl-XL gene expression. Hypoxia (1 h)/re-oxygenation (24 h) have a detrimental effect upon cultured cells by increasing the pro-apoptotic, bax gene and protein expression. Bcl-2 or bcl-XL gene delivery resulted in a significant increase in and the cellular levels of the corresponding mRNAs and proteins. Bcl-2 gene augmented the nestin gene and protein expression which has been compromised previously by the hypoxic event. Similarly c-fos mRNA and protein expression decreased significantly after hypoxia, while the anti-apoptotic gene treatment normalized c-fos expression. Synapsin-1 gene or protein expression remained about on the same level under normoxic conditions or following hypoxia after gene treatment. We can conclude that anti-apoptotic gene transfers activate neuronal plasticity proteins nestin and c-fos. This link on anti-apoptotic proteins and cell plasticity is a new finding.

Original languageEnglish
Pages (from-to)349-353
Number of pages5
JournalNeurochemistry International
Volume55
Issue number5
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Proto-Oncogene Proteins c-fos
Neuronal Plasticity
PC12 Cells
Genetic Therapy
Synapsins
Genes
bcl-2 Genes
Gene Expression
Nestin
Proteins
fos Genes
bcl-2-Associated X Protein
Messenger RNA
Apoptosis Regulatory Proteins
Cultured Cells
Hypoxia
Apoptosis
Therapeutics

Keywords

  • Argon-gas hypoxia
  • Bcl-2
  • Bcl-XL gene transfer
  • Neural plasticity

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Bcl-2 or bcl-XL gene therapy increases neural plasticity proteins nestin and c-fos expression in PC12 cells. / Gal, Aniko; Pentelenyi, Klara; Remenyi, Viktoria; Wappler, Edina A.; Sáfrány, G.; Skopal, Judit; Nagy, Zoltan.

In: Neurochemistry International, Vol. 55, No. 5, 09.2009, p. 349-353.

Research output: Contribution to journalArticle

Gal, Aniko ; Pentelenyi, Klara ; Remenyi, Viktoria ; Wappler, Edina A. ; Sáfrány, G. ; Skopal, Judit ; Nagy, Zoltan. / Bcl-2 or bcl-XL gene therapy increases neural plasticity proteins nestin and c-fos expression in PC12 cells. In: Neurochemistry International. 2009 ; Vol. 55, No. 5. pp. 349-353.
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