Bcl-2 antisense oligonucleotide inhibits the proliferation of childhood leukemia/lymphoma cells of the B-cell lineage

István Szegedi, Klára Katona, András Horváth, Anna Molnár, J. Aradi, C. Kiss

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An 18-mer phosphorothioate bcl-2 atisense oligonucleotide (ASO) inhibited colony formation of three B-cell leukemia/lymphoma cell lines in a dose dependent manner in the range of 0.125-0.5 μmol/l. The srcambled cogener had no detectable effect. A decrease in BCL-2 protein and apoptotic DNA fragmentation was detected in the studied cell lines and primary blast cells of two children with acute lymphoblastic leukemia. Neither BCL-2 protein level, nor DNA integrity was affected by the scrambled control indicating the specific effect ASO. As far as we know, this is the first report on the effects of bcl-2 ASO on childhood leukemia/lymphoma cell samples.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalPathology and Oncology Research
Volume14
Issue number3
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Antisense Oligonucleotides
Cell Lineage
Oligonucleotides
Lymphoma
Leukemia
B-Lymphocytes
B-Cell Leukemia
Cell Line
B-Cell Lymphoma
DNA Fragmentation
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Proteins
DNA

Keywords

  • Antisense oligonucleotide
  • BCL-2
  • Childhood
  • Leukemia
  • Lymphoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Bcl-2 antisense oligonucleotide inhibits the proliferation of childhood leukemia/lymphoma cells of the B-cell lineage. / Szegedi, István; Katona, Klára; Horváth, András; Molnár, Anna; Aradi, J.; Kiss, C.

In: Pathology and Oncology Research, Vol. 14, No. 3, 09.2008, p. 275-279.

Research output: Contribution to journalArticle

Szegedi, István ; Katona, Klára ; Horváth, András ; Molnár, Anna ; Aradi, J. ; Kiss, C. / Bcl-2 antisense oligonucleotide inhibits the proliferation of childhood leukemia/lymphoma cells of the B-cell lineage. In: Pathology and Oncology Research. 2008 ; Vol. 14, No. 3. pp. 275-279.
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