Bait region-thiol ester mapping in human α2-macroglobulin

Peter G W Gettins, Brenda Crews, Albert H. Beth, K. Hideg

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The separations between aromatic residues in the bait region and nitroxide spin labels attached to the thiol ester-forming residues (Cys949 and Gln952) in human α2-macroglobulin (α2M) have been determined from paramagnetic broadening effects of the spin labels on bait region 1H NMR signals. We found that both the Cys949 and Gln952 residues are within 11-17 Å of the aromatic residues in the bait region, with closer approach of some residues to the Gln952 spin label than to the spin label attached to Cys949. A model of the location of bait regions and thiol esters within an α2M half-molecule is proposed that places the bait regions in the central region of α2M at the interface between the subunits.

Original languageEnglish
Pages (from-to)137-140
Number of pages4
JournalFEBS Letters
Volume367
Issue number2
DOIs
Publication statusPublished - Jun 26 1995

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Spin Labels
Macroglobulins
Sulfhydryl Compounds
Esters
Nuclear magnetic resonance
Molecules

Keywords

  • Bait region
  • Human
  • Nitroxide
  • NMR (H)
  • Paramagnetic mapping
  • Spin label
  • Thiol ester
  • α-Macroglobulin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Bait region-thiol ester mapping in human α2-macroglobulin. / Gettins, Peter G W; Crews, Brenda; Beth, Albert H.; Hideg, K.

In: FEBS Letters, Vol. 367, No. 2, 26.06.1995, p. 137-140.

Research output: Contribution to journalArticle

Gettins, Peter G W ; Crews, Brenda ; Beth, Albert H. ; Hideg, K. / Bait region-thiol ester mapping in human α2-macroglobulin. In: FEBS Letters. 1995 ; Vol. 367, No. 2. pp. 137-140.
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