Bacterial triterpenoids of the hopane series from the methanotrophic bacteria Methylocaldum spp. Phylogenetic implications and first evidence for an unsaturated aminobacteriohopanepolyol

Jelena H. Cvejic, Levente Bodrossy, K. Kovács, Michel Rohmer

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The hopanoid content of the two methanotrophic bacteria Methylocaldum szegediense and Methylocaldum tepidum was investigated. 35-Aminobacteriohopane-30R,31R,32R,33S,34S-pentol and its 3β-methyl homologue were present in both strains. In M. tepidum, they were accompanied by 35-aminobacteriohopane-31R,32R,33S,34S-tetrol and its 3β-methyl homologue. The side chain structure was identical to those previously reported from two other obligate methanotrophs, Methylococcus capsulatus and Methylomonas methanica. The two Methylocaldum species shared with the Methylococcus species the presence of 3β-methylhopanoid as well as of a hopanoid releasing adiantol upon H5IO6/NaBH4 treatment. A rare feature was in addition found in M. szegediense. The saturated hopanoids were accompanied by an unsaturated aminobacteriohopanepentol with a Δ11 double bond. Comparison of the hopanoid fingerprints was in accordance with the close phylogenetic relationship of Methylococcus and Methylocaldum. The major difference was the absence of sterols in Methylocaldum which were always detected in the Methylococcus species. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)361-365
Number of pages5
JournalFEMS Microbiology Letters
Volume182
Issue number2
DOIs
Publication statusPublished - Jan 15 2000

Fingerprint

Methylococcus
Bacteria
Methylomonas
Methylococcus capsulatus
Dermatoglyphics
Sterols
hopane

Keywords

  • Chemotaxonomy
  • Hopanoid
  • Methanotrophic bacterium
  • Methylocaldum szegediense
  • Methylocaldum tepidum
  • Triterpenoid

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Bacterial triterpenoids of the hopane series from the methanotrophic bacteria Methylocaldum spp. Phylogenetic implications and first evidence for an unsaturated aminobacteriohopanepolyol. / Cvejic, Jelena H.; Bodrossy, Levente; Kovács, K.; Rohmer, Michel.

In: FEMS Microbiology Letters, Vol. 182, No. 2, 15.01.2000, p. 361-365.

Research output: Contribution to journalArticle

@article{52161094bb6243d8949d9becc5d90043,
title = "Bacterial triterpenoids of the hopane series from the methanotrophic bacteria Methylocaldum spp.: Phylogenetic implications and first evidence for an unsaturated aminobacteriohopanepolyol",
abstract = "The hopanoid content of the two methanotrophic bacteria Methylocaldum szegediense and Methylocaldum tepidum was investigated. 35-Aminobacteriohopane-30R,31R,32R,33S,34S-pentol and its 3β-methyl homologue were present in both strains. In M. tepidum, they were accompanied by 35-aminobacteriohopane-31R,32R,33S,34S-tetrol and its 3β-methyl homologue. The side chain structure was identical to those previously reported from two other obligate methanotrophs, Methylococcus capsulatus and Methylomonas methanica. The two Methylocaldum species shared with the Methylococcus species the presence of 3β-methylhopanoid as well as of a hopanoid releasing adiantol upon H5IO6/NaBH4 treatment. A rare feature was in addition found in M. szegediense. The saturated hopanoids were accompanied by an unsaturated aminobacteriohopanepentol with a Δ11 double bond. Comparison of the hopanoid fingerprints was in accordance with the close phylogenetic relationship of Methylococcus and Methylocaldum. The major difference was the absence of sterols in Methylocaldum which were always detected in the Methylococcus species. Copyright (C) 2000 Federation of European Microbiological Societies.",
keywords = "Chemotaxonomy, Hopanoid, Methanotrophic bacterium, Methylocaldum szegediense, Methylocaldum tepidum, Triterpenoid",
author = "Cvejic, {Jelena H.} and Levente Bodrossy and K. Kov{\'a}cs and Michel Rohmer",
year = "2000",
month = "1",
day = "15",
doi = "10.1016/S0378-1097(99)00610-2",
language = "English",
volume = "182",
pages = "361--365",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Bacterial triterpenoids of the hopane series from the methanotrophic bacteria Methylocaldum spp.

T2 - Phylogenetic implications and first evidence for an unsaturated aminobacteriohopanepolyol

AU - Cvejic, Jelena H.

AU - Bodrossy, Levente

AU - Kovács, K.

AU - Rohmer, Michel

PY - 2000/1/15

Y1 - 2000/1/15

N2 - The hopanoid content of the two methanotrophic bacteria Methylocaldum szegediense and Methylocaldum tepidum was investigated. 35-Aminobacteriohopane-30R,31R,32R,33S,34S-pentol and its 3β-methyl homologue were present in both strains. In M. tepidum, they were accompanied by 35-aminobacteriohopane-31R,32R,33S,34S-tetrol and its 3β-methyl homologue. The side chain structure was identical to those previously reported from two other obligate methanotrophs, Methylococcus capsulatus and Methylomonas methanica. The two Methylocaldum species shared with the Methylococcus species the presence of 3β-methylhopanoid as well as of a hopanoid releasing adiantol upon H5IO6/NaBH4 treatment. A rare feature was in addition found in M. szegediense. The saturated hopanoids were accompanied by an unsaturated aminobacteriohopanepentol with a Δ11 double bond. Comparison of the hopanoid fingerprints was in accordance with the close phylogenetic relationship of Methylococcus and Methylocaldum. The major difference was the absence of sterols in Methylocaldum which were always detected in the Methylococcus species. Copyright (C) 2000 Federation of European Microbiological Societies.

AB - The hopanoid content of the two methanotrophic bacteria Methylocaldum szegediense and Methylocaldum tepidum was investigated. 35-Aminobacteriohopane-30R,31R,32R,33S,34S-pentol and its 3β-methyl homologue were present in both strains. In M. tepidum, they were accompanied by 35-aminobacteriohopane-31R,32R,33S,34S-tetrol and its 3β-methyl homologue. The side chain structure was identical to those previously reported from two other obligate methanotrophs, Methylococcus capsulatus and Methylomonas methanica. The two Methylocaldum species shared with the Methylococcus species the presence of 3β-methylhopanoid as well as of a hopanoid releasing adiantol upon H5IO6/NaBH4 treatment. A rare feature was in addition found in M. szegediense. The saturated hopanoids were accompanied by an unsaturated aminobacteriohopanepentol with a Δ11 double bond. Comparison of the hopanoid fingerprints was in accordance with the close phylogenetic relationship of Methylococcus and Methylocaldum. The major difference was the absence of sterols in Methylocaldum which were always detected in the Methylococcus species. Copyright (C) 2000 Federation of European Microbiological Societies.

KW - Chemotaxonomy

KW - Hopanoid

KW - Methanotrophic bacterium

KW - Methylocaldum szegediense

KW - Methylocaldum tepidum

KW - Triterpenoid

UR - http://www.scopus.com/inward/record.url?scp=0033986814&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033986814&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(99)00610-2

DO - 10.1016/S0378-1097(99)00610-2

M3 - Article

C2 - 10620693

AN - SCOPUS:0033986814

VL - 182

SP - 361

EP - 365

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -