Bacterial adsorption onto monolayer ferromagnetic nanofilms

Farahnaz Ansari, Masoud Kavosh, Robert Horvath, Mohammad Reza Ghalamboran, Jeremy J. Ramsden

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In order to be able to optimize the decoration of bacteria with magnetic nanoparticles, we have developed a methodology for investigating the attachment of nanoparticles to the surface of bacteria by depositing a thin layer of the nanoparticles onto a Si(Ti)O 2 waveguide surface and measuring the bacterial (Rhodococcus erythropolis IGTS8) attachment kinetics under hydrodynamically controlled conditions. This is done by analysing the perturbation of the optical evanescent field generated by light guided along the substratum using optical waveguide lightmode spectroscopy (OWLS), with which the number of deposited particles and attached bacteria could be precisely and accurately determined. Despite the strong attachment implied by the positive surface charge of the nanoparticles and the negatively charged surface of the bacteria, the bacteria are initially attached reversibly. A criterion for ensuring that mixing of the nanoparticles and the bacteria is prolonged sufficiently for effective decoration to take place is proposed.

Original languageEnglish
Pages (from-to)119-122
Number of pages4
JournalJournal of Bionanoscience
Volume4
Issue number1-2
DOIs
Publication statusPublished - Jun 1 2010

Keywords

  • Adsorption
  • Bacteria
  • Kinetics
  • Magnetic Nanoparticles
  • Optical Waveguide Lightmode Spectroscopy

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biomedical Engineering

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  • Cite this

    Ansari, F., Kavosh, M., Horvath, R., Ghalamboran, M. R., & Ramsden, J. J. (2010). Bacterial adsorption onto monolayer ferromagnetic nanofilms. Journal of Bionanoscience, 4(1-2), 119-122. https://doi.org/10.1166/jbns.2010.1025