Bacterial activities in the sediment of Lake Velencei, Hungary

A. Borsodi, Péter Vladár, Gábor Cech, Gábor Gedeon, Bánk Beszteri, Adrienn Micsinai, Mária N. Reskóné, K. Márialigeti

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Lake Velencei (Hungary) is one of the westernmost shallow soda lakes, extending from Eastern Europe to the Carpatian basin. The spatial and temporal distribution of the sediment microbiota, the metabolic potential of bacterial communities and the species composition of the genera Bacillus and Clostridium, as well as sulphate-reducing bacteria (SRB) were investigated regarding the close interactions between the lake sediment and the overlaying water column, the special water chemical parameters, and the extensive reed coverage of the lake. Aerobic microbial activities were tested with community-level physiological profiling (CLPP) using BIOLOG microplates. The quantification of the anaerobic fermentative and sulphate-reducing bacteria was done by the MPN (Most Probable Number) method. The cultivation of bacteria adapted to the special physico-chemical characteristics of the lake was carried out employing selective media. Multivariate analysis of CLPP data indicated that the microbial communities of the sediment separated from that of the water and showed seasonal variations of the utilised carbon sources. The results of the MPN demonstrated that the counts of the fermentative and sulphate-reducing bacteria in the reed rhizosphere were about one order higher than in the sediment. Among the isolated bacterial strains, a large number were characterised as facultative or obligate alkaliphilic and also moderately halophilic. The partial sequencing of 16S rDNA of the selected representatives resulted in species of aerobic bacteria, such as Bacillus pseudofirmus, B. halmapalus, B. cohnii, B. (Marinibacillus) marinus, and anaerobes, such as Clostridium putrificum - sporogenes, C. scatologenes, C. bifermentans, Desulfotomaculum guttoideum, Desulfovibrio alcoholivorans, and Desulfovibrio burkinensis.

Original languageEnglish
Pages (from-to)721-728
Number of pages8
JournalHydrobiologia
Volume506-509
DOIs
Publication statusPublished - Nov 15 2003

Fingerprint

Hungary
microbial activity
sulfate-reducing bacterium
sulfate-reducing bacteria
lakes
sediments
lake
sediment
Bacillus halmapalus
Desulfovibrio burkinensis
Clostridium scatologenes
Bacillus cohnii
Bacillus pseudofirmus
Clostridium bifermentans
Desulfotomaculum
bacterium
Desulfovibrio
microplate
Clostridium botulinum
multivariate analysis

Keywords

  • 16S rDNA
  • Alkaliphilic Bacillus
  • BIOLOG
  • Clostridia
  • Soda lake sediment
  • Sulphate-reducing bacteria

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Bacterial activities in the sediment of Lake Velencei, Hungary. / Borsodi, A.; Vladár, Péter; Cech, Gábor; Gedeon, Gábor; Beszteri, Bánk; Micsinai, Adrienn; Reskóné, Mária N.; Márialigeti, K.

In: Hydrobiologia, Vol. 506-509, 15.11.2003, p. 721-728.

Research output: Contribution to journalArticle

Borsodi, A, Vladár, P, Cech, G, Gedeon, G, Beszteri, B, Micsinai, A, Reskóné, MN & Márialigeti, K 2003, 'Bacterial activities in the sediment of Lake Velencei, Hungary', Hydrobiologia, vol. 506-509, pp. 721-728. https://doi.org/10.1023/B:HYDR.0000008586.30395.f2
Borsodi A, Vladár P, Cech G, Gedeon G, Beszteri B, Micsinai A et al. Bacterial activities in the sediment of Lake Velencei, Hungary. Hydrobiologia. 2003 Nov 15;506-509:721-728. https://doi.org/10.1023/B:HYDR.0000008586.30395.f2
Borsodi, A. ; Vladár, Péter ; Cech, Gábor ; Gedeon, Gábor ; Beszteri, Bánk ; Micsinai, Adrienn ; Reskóné, Mária N. ; Márialigeti, K. / Bacterial activities in the sediment of Lake Velencei, Hungary. In: Hydrobiologia. 2003 ; Vol. 506-509. pp. 721-728.
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