Bacteria repellent layer made of flagellin

Boglarka Kovacs, Daniel Patko, Agnes Klein, Balazs Kakasi, Andras Saftics, Sandor Kurunczi, F. Vonderviszt, R. Horváth

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The development of bacteria repellent surface coatings is critical in various fields ranging from biosensing to health care, biotechnology and food production. In the present study we exploit that the protein flagellin rapidly forms a dense and oriented monolayer on hydrophobic surfaces upon adsorption from aqueous solution. This oriented layer mimics the surface of bacterial flagellar filaments and has excellent bacteria repellent properties. In situ OWLS (Optical Waveguide Lightmode Spectroscopy) measurements were used to monitor on-line both the formation of the protein layer on the silanized sensor surface and subsequent bacterial adhesion. The adhered cells were also visualized by fluorescent microscopy and the formed protein film was characterized by AFM (Atomic Force Microscopy). In parallel control experiments, the adherence of bacteria was measured on bare hydrophobic surfaces as well. Both OWLS and microscopy results well confirmed that the flagellin coating drastically reduced the adhesion of E. coli cells. Therefore, a novel type of bacteria repellent layer made of flagellin is demonstrated.

Original languageEnglish
Pages (from-to)839-845
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume257
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

Flagellin
bacteria
Bacteria
Optical waveguides
proteins
Proteins
optical waveguides
Microscopic examination
adhesion
Adhesion
Spectroscopy
microscopy
coatings
Coatings
biotechnology
Biotechnology
cells
Health care
Escherichia coli
spectroscopy

Keywords

  • Bacteria repellent coating
  • Bacterial adhesion
  • Biomimetic coating
  • Flagellin
  • Optical biosensor
  • OWLS

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Kovacs, B., Patko, D., Klein, A., Kakasi, B., Saftics, A., Kurunczi, S., ... Horváth, R. (2018). Bacteria repellent layer made of flagellin. Sensors and Actuators, B: Chemical, 257, 839-845. https://doi.org/10.1016/j.snb.2017.11.027

Bacteria repellent layer made of flagellin. / Kovacs, Boglarka; Patko, Daniel; Klein, Agnes; Kakasi, Balazs; Saftics, Andras; Kurunczi, Sandor; Vonderviszt, F.; Horváth, R.

In: Sensors and Actuators, B: Chemical, Vol. 257, 01.03.2018, p. 839-845.

Research output: Contribution to journalArticle

Kovacs, B, Patko, D, Klein, A, Kakasi, B, Saftics, A, Kurunczi, S, Vonderviszt, F & Horváth, R 2018, 'Bacteria repellent layer made of flagellin', Sensors and Actuators, B: Chemical, vol. 257, pp. 839-845. https://doi.org/10.1016/j.snb.2017.11.027
Kovacs B, Patko D, Klein A, Kakasi B, Saftics A, Kurunczi S et al. Bacteria repellent layer made of flagellin. Sensors and Actuators, B: Chemical. 2018 Mar 1;257:839-845. https://doi.org/10.1016/j.snb.2017.11.027
Kovacs, Boglarka ; Patko, Daniel ; Klein, Agnes ; Kakasi, Balazs ; Saftics, Andras ; Kurunczi, Sandor ; Vonderviszt, F. ; Horváth, R. / Bacteria repellent layer made of flagellin. In: Sensors and Actuators, B: Chemical. 2018 ; Vol. 257. pp. 839-845.
@article{78bc4bec22ea4f11818b9b16b2a5bcc2,
title = "Bacteria repellent layer made of flagellin",
abstract = "The development of bacteria repellent surface coatings is critical in various fields ranging from biosensing to health care, biotechnology and food production. In the present study we exploit that the protein flagellin rapidly forms a dense and oriented monolayer on hydrophobic surfaces upon adsorption from aqueous solution. This oriented layer mimics the surface of bacterial flagellar filaments and has excellent bacteria repellent properties. In situ OWLS (Optical Waveguide Lightmode Spectroscopy) measurements were used to monitor on-line both the formation of the protein layer on the silanized sensor surface and subsequent bacterial adhesion. The adhered cells were also visualized by fluorescent microscopy and the formed protein film was characterized by AFM (Atomic Force Microscopy). In parallel control experiments, the adherence of bacteria was measured on bare hydrophobic surfaces as well. Both OWLS and microscopy results well confirmed that the flagellin coating drastically reduced the adhesion of E. coli cells. Therefore, a novel type of bacteria repellent layer made of flagellin is demonstrated.",
keywords = "Bacteria repellent coating, Bacterial adhesion, Biomimetic coating, Flagellin, Optical biosensor, OWLS",
author = "Boglarka Kovacs and Daniel Patko and Agnes Klein and Balazs Kakasi and Andras Saftics and Sandor Kurunczi and F. Vonderviszt and R. Horv{\'a}th",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.snb.2017.11.027",
language = "English",
volume = "257",
pages = "839--845",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

TY - JOUR

T1 - Bacteria repellent layer made of flagellin

AU - Kovacs, Boglarka

AU - Patko, Daniel

AU - Klein, Agnes

AU - Kakasi, Balazs

AU - Saftics, Andras

AU - Kurunczi, Sandor

AU - Vonderviszt, F.

AU - Horváth, R.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - The development of bacteria repellent surface coatings is critical in various fields ranging from biosensing to health care, biotechnology and food production. In the present study we exploit that the protein flagellin rapidly forms a dense and oriented monolayer on hydrophobic surfaces upon adsorption from aqueous solution. This oriented layer mimics the surface of bacterial flagellar filaments and has excellent bacteria repellent properties. In situ OWLS (Optical Waveguide Lightmode Spectroscopy) measurements were used to monitor on-line both the formation of the protein layer on the silanized sensor surface and subsequent bacterial adhesion. The adhered cells were also visualized by fluorescent microscopy and the formed protein film was characterized by AFM (Atomic Force Microscopy). In parallel control experiments, the adherence of bacteria was measured on bare hydrophobic surfaces as well. Both OWLS and microscopy results well confirmed that the flagellin coating drastically reduced the adhesion of E. coli cells. Therefore, a novel type of bacteria repellent layer made of flagellin is demonstrated.

AB - The development of bacteria repellent surface coatings is critical in various fields ranging from biosensing to health care, biotechnology and food production. In the present study we exploit that the protein flagellin rapidly forms a dense and oriented monolayer on hydrophobic surfaces upon adsorption from aqueous solution. This oriented layer mimics the surface of bacterial flagellar filaments and has excellent bacteria repellent properties. In situ OWLS (Optical Waveguide Lightmode Spectroscopy) measurements were used to monitor on-line both the formation of the protein layer on the silanized sensor surface and subsequent bacterial adhesion. The adhered cells were also visualized by fluorescent microscopy and the formed protein film was characterized by AFM (Atomic Force Microscopy). In parallel control experiments, the adherence of bacteria was measured on bare hydrophobic surfaces as well. Both OWLS and microscopy results well confirmed that the flagellin coating drastically reduced the adhesion of E. coli cells. Therefore, a novel type of bacteria repellent layer made of flagellin is demonstrated.

KW - Bacteria repellent coating

KW - Bacterial adhesion

KW - Biomimetic coating

KW - Flagellin

KW - Optical biosensor

KW - OWLS

UR - http://www.scopus.com/inward/record.url?scp=85034048661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034048661&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2017.11.027

DO - 10.1016/j.snb.2017.11.027

M3 - Article

AN - SCOPUS:85034048661

VL - 257

SP - 839

EP - 845

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

ER -