Bacillus alkalisediminis sp. nov., an alkaliphilic and moderately halophilic bacterium isolated from sediment of extremely shallow soda ponds

A. Borsodi, Beatrix Pollák, Zsuzsa Kéki, Anna Rusznyák, A. Kovács, Cathrin Spröer, Peter Schumann, K. Márialigeti, E. Tóth

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Alkaliphilic strains characterized by optimal growth at pH 9.0 and 5% (w/v) NaCl designated K1-25 T and H3-93 were isolated from extremely shallow soda ponds located in Hungary. Cells of both strains were Gram-stain-positive, non-motile, straight rods and formed central, ellipsoidal endospores with swollen sporangia. The isolates were aerobic, catalase-positive, oxidase-negative and contained a peptidoglycan of type A1γ based on meso-diaminopimelic acid. In both strains, menaquinone-7 (MK-7) was the predominant isoprenoid quinone and the major cellular fatty acids were anteiso-C 15: 0 and iso-C 15: 0. The DNA G+C contents of strains K1-25 T and H3-93 were 39.0 and 36.3 mol%, respectively. 16S rRNA gene sequence-based phylogenetic analysis revealed 99.2% similarity between strains K1-25 T and H3-93 and the novel isolates had the highest similarities to Bacillus akibai 1139 T (97.8 and 98.3%, respectively), Bacillus wakoensis N-1 T (97.0 and 97.4%), Bacillus okhensis Kh10-101 T (97.1 and 97.4%) and Bacillus krulwichiae AM31D T (96.9 and 97.1%). DNA-DNA hybridization between our strains and the type strains of closely related Bacillus species was lower than 70%. Although DNA-DNA hybridization between strains K1-25 T and H3-93 was 27%, the phenotypic and chemotaxonomic data did not support the differentiation of these two strains into separate species. Therefore, they represent genomovars of a novel species, for which the name Bacillus alkalisediminis sp. nov. is proposed. The type strain is K1-25 T (5DSM 21670 T 5NCAIM B02301 T).

Original languageEnglish
Pages (from-to)1880-1886
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume61
Issue number8
DOIs
Publication statusPublished - Aug 2011

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Bacillus (bacteria)
Bacillus
pond
Bacteria
DNA
sediments
bacterium
new species
bacteria
sediment
Sporangia
Diaminopimelic Acid
isoprenoid
nucleic acid hybridization
Peptidoglycan
Hungary
Terpenes
Base Composition
rRNA Genes
fatty acid

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Bacillus alkalisediminis sp. nov., an alkaliphilic and moderately halophilic bacterium isolated from sediment of extremely shallow soda ponds. / Borsodi, A.; Pollák, Beatrix; Kéki, Zsuzsa; Rusznyák, Anna; Kovács, A.; Spröer, Cathrin; Schumann, Peter; Márialigeti, K.; Tóth, E.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 61, No. 8, 08.2011, p. 1880-1886.

Research output: Contribution to journalArticle

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abstract = "Alkaliphilic strains characterized by optimal growth at pH 9.0 and 5{\%} (w/v) NaCl designated K1-25 T and H3-93 were isolated from extremely shallow soda ponds located in Hungary. Cells of both strains were Gram-stain-positive, non-motile, straight rods and formed central, ellipsoidal endospores with swollen sporangia. The isolates were aerobic, catalase-positive, oxidase-negative and contained a peptidoglycan of type A1γ based on meso-diaminopimelic acid. In both strains, menaquinone-7 (MK-7) was the predominant isoprenoid quinone and the major cellular fatty acids were anteiso-C 15: 0 and iso-C 15: 0. The DNA G+C contents of strains K1-25 T and H3-93 were 39.0 and 36.3 mol{\%}, respectively. 16S rRNA gene sequence-based phylogenetic analysis revealed 99.2{\%} similarity between strains K1-25 T and H3-93 and the novel isolates had the highest similarities to Bacillus akibai 1139 T (97.8 and 98.3{\%}, respectively), Bacillus wakoensis N-1 T (97.0 and 97.4{\%}), Bacillus okhensis Kh10-101 T (97.1 and 97.4{\%}) and Bacillus krulwichiae AM31D T (96.9 and 97.1{\%}). DNA-DNA hybridization between our strains and the type strains of closely related Bacillus species was lower than 70{\%}. Although DNA-DNA hybridization between strains K1-25 T and H3-93 was 27{\%}, the phenotypic and chemotaxonomic data did not support the differentiation of these two strains into separate species. Therefore, they represent genomovars of a novel species, for which the name Bacillus alkalisediminis sp. nov. is proposed. The type strain is K1-25 T (5DSM 21670 T 5NCAIM B02301 T).",
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AU - Borsodi, A.

AU - Pollák, Beatrix

AU - Kéki, Zsuzsa

AU - Rusznyák, Anna

AU - Kovács, A.

AU - Spröer, Cathrin

AU - Schumann, Peter

AU - Márialigeti, K.

AU - Tóth, E.

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AB - Alkaliphilic strains characterized by optimal growth at pH 9.0 and 5% (w/v) NaCl designated K1-25 T and H3-93 were isolated from extremely shallow soda ponds located in Hungary. Cells of both strains were Gram-stain-positive, non-motile, straight rods and formed central, ellipsoidal endospores with swollen sporangia. The isolates were aerobic, catalase-positive, oxidase-negative and contained a peptidoglycan of type A1γ based on meso-diaminopimelic acid. In both strains, menaquinone-7 (MK-7) was the predominant isoprenoid quinone and the major cellular fatty acids were anteiso-C 15: 0 and iso-C 15: 0. The DNA G+C contents of strains K1-25 T and H3-93 were 39.0 and 36.3 mol%, respectively. 16S rRNA gene sequence-based phylogenetic analysis revealed 99.2% similarity between strains K1-25 T and H3-93 and the novel isolates had the highest similarities to Bacillus akibai 1139 T (97.8 and 98.3%, respectively), Bacillus wakoensis N-1 T (97.0 and 97.4%), Bacillus okhensis Kh10-101 T (97.1 and 97.4%) and Bacillus krulwichiae AM31D T (96.9 and 97.1%). DNA-DNA hybridization between our strains and the type strains of closely related Bacillus species was lower than 70%. Although DNA-DNA hybridization between strains K1-25 T and H3-93 was 27%, the phenotypic and chemotaxonomic data did not support the differentiation of these two strains into separate species. Therefore, they represent genomovars of a novel species, for which the name Bacillus alkalisediminis sp. nov. is proposed. The type strain is K1-25 T (5DSM 21670 T 5NCAIM B02301 T).

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