Babesia canis canis in dogs from Hungary

Detection by PCR and sequencing

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Canine babesiosis in Hungary has always been a severe and frequent disease, attributed to infection with Babesia canis transmitted by Dermacentor reticulatus. Identification of the disease agent has been based merely on size and morphology of the intraerythrocytic parasites and no evidence has been found concerning the subspecies (genotype) of B. canis. Therefore, a molecular survey on natural Babesia infection of dogs in Hungary using PCR and sequence analysis was attempted to clarify the subspecies (genotype) and to obtain information on the occurrence of B. canis. A total of 44 blood samples from dogs showing clinical signs of babesiosis were collected. A piroplasm-specific PCR amplifying the partial 18S rRNA gene yielded an approximately 450 bp PCR product in 39 (88.6%) samples. Thirteen positive samples originated from Budapest and 26 from 21 other locations. Five PCR products were chosen randomly for sequencing. The partial 18S rDNA sequences were submitted to GenBank® (accession numbers AY611729; AY611730; AY611731; AY611732 and AY611733). The sequences showed 100% homology to one another or differed by one nucleotide. BLAST search against GenBank® revealed the highest similarity (99.8 or 100%) with Babesia canis canis. The implication of these data, for the further study and diagnosis of canine babesiosis is discussed.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalVeterinary Parasitology
Volume127
Issue number3-4
DOIs
Publication statusPublished - Feb 28 2005

Fingerprint

Babesia
Hungary
Babesiosis
Babesia canis
babesiosis
Dogs
Polymerase Chain Reaction
dogs
Nucleic Acid Databases
Canidae
Genotype
Dermacentor
Dermacentor reticulatus
Piroplasmida
genotype
Ribosomal DNA
Infection
rRNA Genes
sampling
infection

Keywords

  • 18S rDNA
  • Babesia canis canis
  • Dog
  • Hungary
  • PCR

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Parasitology
  • veterinary(all)

Cite this

Babesia canis canis in dogs from Hungary : Detection by PCR and sequencing. / Földvári, G.; Hell, Éva; Farkas, R.

In: Veterinary Parasitology, Vol. 127, No. 3-4, 28.02.2005, p. 221-226.

Research output: Contribution to journalArticle

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