B-type natriuretic peptide limits infarct size in rat isolated hearts via KATP channel opening

Savio P. D'Souza, Derek M. Yellon, Claus Martin, Rainer Schulz, Gerd Heusch, Annamaria Onody, P. Ferdinándy, Gary F. Baxter

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

B-type natriuretic peptide (BNP) has been reported to be released from me myocardium during ischemia. We hypothesized that BNP mediates cardioprotection during ischemia-reperfusion and examined whether exogenous BNP limits myocardial infarction and the potential role of ATP-sensitive potassium (KATP) channel opening. Langendorff-perfused rat hearts underwent 35 min of left coronary artery occlusion and 120 min of reperfusion. The control infarct-to-risk ratio was 44.8 ± 4.4% (means ± SE). BNP perfused 10 min before ischemia limited infarct size in a concentration-dependent manner, with maximal protection observed at 10-8 M (infarct-to-risk ratio: 20.1 ± 5.2%, P <0.01 vs. control), associated with a 2.5-fold elevation of myocardial cGMP above the control value. To examine the role of KATp channel opening, glibenclamide (10-6 M), 5-hydroxydecanoate (5-HD; 10-4 M), or HMR-1098 (10-5 M) was coperfused with BNP (10-8 M). Protection afforded by BNP was abolished by glibenclamide or 5-HD but not by HMR-1098, suggesting the involvement of putative mitochondrial but not sarcolemmal KATP channel opening. We conclude that natriuretic peptide/cGMP/KATP channel signaling may constitute an important injury-limiting mechanism in myocardium.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume284
Issue number5 53-5
Publication statusPublished - May 1 2003

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KATP Channels
Brain Natriuretic Peptide
Ischemia
Glyburide
Reperfusion
Myocardium
Odds Ratio
Natriuretic Peptides
Coronary Occlusion
Coronary Vessels
Myocardial Infarction
Wounds and Injuries

Keywords

  • cGMP
  • Infarct size
  • Ischemia-reperfusion
  • Preconditioning

ASJC Scopus subject areas

  • Physiology

Cite this

D'Souza, S. P., Yellon, D. M., Martin, C., Schulz, R., Heusch, G., Onody, A., ... Baxter, G. F. (2003). B-type natriuretic peptide limits infarct size in rat isolated hearts via KATP channel opening. American Journal of Physiology - Heart and Circulatory Physiology, 284(5 53-5).

B-type natriuretic peptide limits infarct size in rat isolated hearts via KATP channel opening. / D'Souza, Savio P.; Yellon, Derek M.; Martin, Claus; Schulz, Rainer; Heusch, Gerd; Onody, Annamaria; Ferdinándy, P.; Baxter, Gary F.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 284, No. 5 53-5, 01.05.2003.

Research output: Contribution to journalArticle

D'Souza, Savio P. ; Yellon, Derek M. ; Martin, Claus ; Schulz, Rainer ; Heusch, Gerd ; Onody, Annamaria ; Ferdinándy, P. ; Baxter, Gary F. / B-type natriuretic peptide limits infarct size in rat isolated hearts via KATP channel opening. In: American Journal of Physiology - Heart and Circulatory Physiology. 2003 ; Vol. 284, No. 5 53-5.
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