Axonterminal uptake and retrograde axonal transport of labeled amino acids and their incorporation into proteins of neuronal perikarya

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Abstract

The axon terminal uptake and retrograde axonal transport of [3H]leucine and [3H]lysine was investigated. Amino acids were injected into the interpositus nucleus of adult cats, then labeling was studied using light- and electron-microscopic autoradiography. Besides the usual extracellular (terminal) labeling, grains were found in the perikarya of the inferior olive, external cuneate nucleus and the red nucleus. Intra-olivary application of puromycin (known to inhibit protein synthesis) resulted in a significant decrease of the local perikaryonal labeling. In the contralateral inferior olive a diminished incorporation of labeled amino acids into proteins was observed after surgical deafferentation. We conclude that the retrograde axonal transport of amino acids is a useful technique for investigating neuronal pathways and innervation patterns.

Original languageEnglish
Pages (from-to)2035-2046
Number of pages12
JournalNeuroscience
Volume6
Issue number10
DOIs
Publication statusPublished - Oct 1981

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ASJC Scopus subject areas

  • Neuroscience(all)

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