Axial stretching of extremity artery induces reversible hyperpolarization of smooth muscle cell membrane in vivo

E. Monos, G. Raffai, S. J. Contney, W. J. Stekiel, A. W. Cowley

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Circumferential stretch due to increases in pressure induces vascular smooth muscle cell depolarization and contraction known as the myogenic response. The aim of this study was to determine the in vivo effects of axial-longitudinal stretch of the rat saphenous artery (SA) on smooth muscle membrane potential (Em) and on external diameter. Consecutive elongations of the SA were carried out from resting length (L0) in 10% increments up to 140% L0 while changes in membrane potential and diameter were determined in intact and de-endothelized vessels. Axial stretching resulted in a small initial depolarization at 120% of L0 followed by a progressive 20 to 33% hyperpolarizaion of vascular smooth muscle between 130% and 140% of L0. At 140%, an average maximal 10.6 mV reversible hyperpolarization was measured compared to -41.2± 0.49 mV Em at 100% L0. De-endothelialization completely eliminated the hyperpolarization to axial stretching and augmented the reduction of diameter beyond 120% L0. These results indicate that arteries have a mechanism to protect them from vasospasm that could otherwise occur with movements of the extremities.

Original languageEnglish
Pages (from-to)197-206
Number of pages10
JournalActa Physiologica Hungarica
Volume88
Issue number3-4
DOIs
Publication statusPublished - 2001

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Smooth Muscle Myocytes
Extremities
Arteries
Cell Membrane
Vascular Smooth Muscle
Membrane Potentials
Muscle Contraction
Smooth Muscle
Pressure

Keywords

  • Arterial strain
  • Endothelium
  • Smooth muscle
  • Vascular myogenic response

ASJC Scopus subject areas

  • Physiology

Cite this

Axial stretching of extremity artery induces reversible hyperpolarization of smooth muscle cell membrane in vivo. / Monos, E.; Raffai, G.; Contney, S. J.; Stekiel, W. J.; Cowley, A. W.

In: Acta Physiologica Hungarica, Vol. 88, No. 3-4, 2001, p. 197-206.

Research output: Contribution to journalArticle

Monos, E. ; Raffai, G. ; Contney, S. J. ; Stekiel, W. J. ; Cowley, A. W. / Axial stretching of extremity artery induces reversible hyperpolarization of smooth muscle cell membrane in vivo. In: Acta Physiologica Hungarica. 2001 ; Vol. 88, No. 3-4. pp. 197-206.
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