Axial ligation of the high-potential heme center in an Arabidopsis cytochrome b561

Filip Desmet, Alajos Bérczi, László Zimányi, Han Asard, Sabine Van Doorslaer

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Abstract

Arabidopsis has four putative, di-heme cytochrome b561 proteins, including one localized in the tonoplast (TCytb). From a comparative electron paramagnetic resonance (EPR), UV-Vis absorption and resonance Raman study, on wild type, H83A/H156A-TCytb and H83L/H156L-TCytb double mutants, it follows that the H83 and H156 residues are binding one of the two hemes. These measurements show that the high-potential heme site is situated at the cytoplasmic side of the membrane and allow the unambiguous differentiation between two models on the heme localization in cytochrome b561 proteins.

Original languageEnglish
Pages (from-to)545-548
Number of pages4
JournalFEBS letters
Volume585
Issue number3
DOIs
Publication statusPublished - Feb 4 2011

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Keywords

  • Cytochrome b561
  • Double mutant
  • EPR
  • Optical absorption spectroscopy
  • Resonance Raman spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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