Auxin autotrophic tobacco callus tissues resist oxidative stress: The importance of glutathione S-transferase and glutathione peroxidase activities in auxin heterotrophic and autotrophic calli

J. Csiszár, Margit Szabó, L. Erdei, László Márton, F. Horváth, I. Tari

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Auxin autotrophic and heterotrophic tobacco callus lines were grown on MS medium with or without 100 mmol/L NaCl and growth and some of the stress-related activities, such as GPX, SOD, CAT, GST, GSH-PX, as well as the concentration of ethylene and H2O2, were measured and compared with each other. The auxin autotrophic calli grew slower, however, on the NaCl-containing medium the growth rate was higher than that of the heterotrophic cultures after two weeks of culturing. The stress-related ethylene production was lower in the autotrophic cultures and, contrary to the heterotrophic tissues, its level did not change significantly upon NaCl treatment. The guaiacol peroxidase (GPX) activities were higher in the autotrophic tissues in all cell fractions regardless of the presence of NaCl. Treated with NaCl, the GPX activities elevated in the soluble and covalently-bound fractions in the heterotrophic calli, but were not further increased in the autotrophic line. SOD and CAT activities were higher in the heterotrophic tissues, and were increased further by 100 mmol/L NaCl treatment. The GST and GSH-PX activities were higher in the autotrophic line, which might explain their enhanced stress tolerance. In the autotrophic tissues, the elevated antioxidant activities led to reduced levels of H2O2 and malondialdehyde; under mild NaCl stress, these levels decreased further. The lower growth rate and the effective protection against NaCl stress-induced oxidative damage of the autotrophic line can be explained by the cell wall-bound peroxidase and GSH-PX activities in the auxin autotrophic tissues. Their maintained growth rate indicates that the autotropic cultures were more resistant to exogenous H2O2.

Original languageEnglish
Pages (from-to)691-699
Number of pages9
JournalJournal of Plant Physiology
Volume161
Issue number6
DOIs
Publication statusPublished - 2004

Fingerprint

Indoleacetic Acids
Bony Callus
Glutathione Peroxidase
Glutathione Transferase
glutathione transferase
glutathione peroxidase
Tobacco
auxins
callus
Oxidative Stress
tobacco
oxidative stress
peroxidase
Growth
superoxide dismutase
Malondialdehyde
ethylene production
stress tolerance
Cell Wall
malondialdehyde

Keywords

  • Auxin-dependent and independent calli
  • Ethylene
  • Glutathione peroxidase
  • Oxidative stress resistance
  • Scavenging enzymes

ASJC Scopus subject areas

  • Plant Science

Cite this

@article{59b38658213049d9be07333b1a403dde,
title = "Auxin autotrophic tobacco callus tissues resist oxidative stress: The importance of glutathione S-transferase and glutathione peroxidase activities in auxin heterotrophic and autotrophic calli",
abstract = "Auxin autotrophic and heterotrophic tobacco callus lines were grown on MS medium with or without 100 mmol/L NaCl and growth and some of the stress-related activities, such as GPX, SOD, CAT, GST, GSH-PX, as well as the concentration of ethylene and H2O2, were measured and compared with each other. The auxin autotrophic calli grew slower, however, on the NaCl-containing medium the growth rate was higher than that of the heterotrophic cultures after two weeks of culturing. The stress-related ethylene production was lower in the autotrophic cultures and, contrary to the heterotrophic tissues, its level did not change significantly upon NaCl treatment. The guaiacol peroxidase (GPX) activities were higher in the autotrophic tissues in all cell fractions regardless of the presence of NaCl. Treated with NaCl, the GPX activities elevated in the soluble and covalently-bound fractions in the heterotrophic calli, but were not further increased in the autotrophic line. SOD and CAT activities were higher in the heterotrophic tissues, and were increased further by 100 mmol/L NaCl treatment. The GST and GSH-PX activities were higher in the autotrophic line, which might explain their enhanced stress tolerance. In the autotrophic tissues, the elevated antioxidant activities led to reduced levels of H2O2 and malondialdehyde; under mild NaCl stress, these levels decreased further. The lower growth rate and the effective protection against NaCl stress-induced oxidative damage of the autotrophic line can be explained by the cell wall-bound peroxidase and GSH-PX activities in the auxin autotrophic tissues. Their maintained growth rate indicates that the autotropic cultures were more resistant to exogenous H2O2.",
keywords = "Auxin-dependent and independent calli, Ethylene, Glutathione peroxidase, Oxidative stress resistance, Scavenging enzymes",
author = "J. Csisz{\'a}r and Margit Szab{\'o} and L. Erdei and L{\'a}szl{\'o} M{\'a}rton and F. Horv{\'a}th and I. Tari",
year = "2004",
doi = "10.1078/0176-1617-01071",
language = "English",
volume = "161",
pages = "691--699",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "6",

}

TY - JOUR

T1 - Auxin autotrophic tobacco callus tissues resist oxidative stress

T2 - The importance of glutathione S-transferase and glutathione peroxidase activities in auxin heterotrophic and autotrophic calli

AU - Csiszár, J.

AU - Szabó, Margit

AU - Erdei, L.

AU - Márton, László

AU - Horváth, F.

AU - Tari, I.

PY - 2004

Y1 - 2004

N2 - Auxin autotrophic and heterotrophic tobacco callus lines were grown on MS medium with or without 100 mmol/L NaCl and growth and some of the stress-related activities, such as GPX, SOD, CAT, GST, GSH-PX, as well as the concentration of ethylene and H2O2, were measured and compared with each other. The auxin autotrophic calli grew slower, however, on the NaCl-containing medium the growth rate was higher than that of the heterotrophic cultures after two weeks of culturing. The stress-related ethylene production was lower in the autotrophic cultures and, contrary to the heterotrophic tissues, its level did not change significantly upon NaCl treatment. The guaiacol peroxidase (GPX) activities were higher in the autotrophic tissues in all cell fractions regardless of the presence of NaCl. Treated with NaCl, the GPX activities elevated in the soluble and covalently-bound fractions in the heterotrophic calli, but were not further increased in the autotrophic line. SOD and CAT activities were higher in the heterotrophic tissues, and were increased further by 100 mmol/L NaCl treatment. The GST and GSH-PX activities were higher in the autotrophic line, which might explain their enhanced stress tolerance. In the autotrophic tissues, the elevated antioxidant activities led to reduced levels of H2O2 and malondialdehyde; under mild NaCl stress, these levels decreased further. The lower growth rate and the effective protection against NaCl stress-induced oxidative damage of the autotrophic line can be explained by the cell wall-bound peroxidase and GSH-PX activities in the auxin autotrophic tissues. Their maintained growth rate indicates that the autotropic cultures were more resistant to exogenous H2O2.

AB - Auxin autotrophic and heterotrophic tobacco callus lines were grown on MS medium with or without 100 mmol/L NaCl and growth and some of the stress-related activities, such as GPX, SOD, CAT, GST, GSH-PX, as well as the concentration of ethylene and H2O2, were measured and compared with each other. The auxin autotrophic calli grew slower, however, on the NaCl-containing medium the growth rate was higher than that of the heterotrophic cultures after two weeks of culturing. The stress-related ethylene production was lower in the autotrophic cultures and, contrary to the heterotrophic tissues, its level did not change significantly upon NaCl treatment. The guaiacol peroxidase (GPX) activities were higher in the autotrophic tissues in all cell fractions regardless of the presence of NaCl. Treated with NaCl, the GPX activities elevated in the soluble and covalently-bound fractions in the heterotrophic calli, but were not further increased in the autotrophic line. SOD and CAT activities were higher in the heterotrophic tissues, and were increased further by 100 mmol/L NaCl treatment. The GST and GSH-PX activities were higher in the autotrophic line, which might explain their enhanced stress tolerance. In the autotrophic tissues, the elevated antioxidant activities led to reduced levels of H2O2 and malondialdehyde; under mild NaCl stress, these levels decreased further. The lower growth rate and the effective protection against NaCl stress-induced oxidative damage of the autotrophic line can be explained by the cell wall-bound peroxidase and GSH-PX activities in the auxin autotrophic tissues. Their maintained growth rate indicates that the autotropic cultures were more resistant to exogenous H2O2.

KW - Auxin-dependent and independent calli

KW - Ethylene

KW - Glutathione peroxidase

KW - Oxidative stress resistance

KW - Scavenging enzymes

UR - http://www.scopus.com/inward/record.url?scp=3042803769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042803769&partnerID=8YFLogxK

U2 - 10.1078/0176-1617-01071

DO - 10.1078/0176-1617-01071

M3 - Article

C2 - 15266716

AN - SCOPUS:3042803769

VL - 161

SP - 691

EP - 699

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 6

ER -