Auxin autotrophic tobacco calli with modified aldehyde oxidase isoenzyme activities show enhanced abiotic stress tolerance

Jolán Csiszár, Margit Szabó, Dorica Botau, László Erdei, Irma Tari

Research output: Contribution to journalArticle

Abstract

The growth, abiotic stress resistance and aldehyde oxidase (AO) activities of auxin autotrophic and heterotrophic tobacco calli were compared. The auxin-independent autotrophic calli maintained their growth rate even at 35°C temperatures, showed enhanced abiotic stress resistance in the presence of 50-300 mM NaCl, 0.1-5 mM KNO2 or 0.1-10 mM H2O2. 0.1-5 mM H2O2 caused a higher increase in glutathione S-transferase (GST) and glutathione peroxidase (GPOX) activities of heterotrophic calli, however these enzymes worked in an elevated level in autotrophic lines under control circumstances and were induced differently under oxidative stress, indicating an altered signalling mechanism. AO activity could be detected by activity staining after native PAGE with indole-3-acetaldehyde (IAAld) substrate in both calli, which means that the enzyme catalyzing the last step in IAA biosynthesis is present in both tissues. Contrary to heterotrophic calli, in the auxin autotrophic cultures an isoenzyme with low mobility (AO1) was detectable. 100 mM NaCl enhanced the AO1 activity and a new isoenzyme (AO2) was observed. The increase of the activities of these isoenzymes were higher in the autotrophic lines suggesting that the enhanced IAA biosynthesis can play a role in the recovery of growth under stress conditions.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalActa Biologica Szegediensis
Volume51
Issue number1
Publication statusPublished - Jan 1 2007

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Keywords

  • Aldehyde oxidase
  • Auxin heterotrophic and autotrophic calli
  • Glutathione S-transferase and peroxidase
  • Oxidative stress resistance

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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