Autophagy is required for zebrafish caudal fin regeneration

M. Varga, M. Sass, D. Papp, K. Takács-Vellai, J. Kobolak, A. Dinnyés, D. J. Klionsky, T. Vellai

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Regeneration is the ability of multicellular organisms to replace damaged tissues and regrow lost body parts. This process relies on cell fate transformation that involves changes in gene expression as well as in the composition of the cytoplasmic compartment, and exhibits a characteristic age-related decline. Here, we present evidence that genetic and pharmacological inhibition of autophagy - a lysosome-mediated self-degradation process of eukaryotic cells, which has been implicated in extensive cellular remodelling and aging - impairs the regeneration of amputated caudal fins in the zebrafish (Danio rerio). Thus, autophagy is required for injury-induced tissue renewal. We further show that upregulation of autophagy in the regeneration zone occurs downstream of mitogen-activated protein kinase/extracellular signal-regulated kinase signalling to protect cells from undergoing apoptosis and enable cytosolic restructuring underlying terminal cell fate determination. This novel cellular function of the autophagic process in regeneration implies that the role of cellular self-digestion in differentiation and tissue patterning is more fundamental than previously thought.

Original languageEnglish
Pages (from-to)547-556
Number of pages10
JournalCell Death and Differentiation
Volume21
Issue number4
DOIs
Publication statusPublished - Apr 2014

Fingerprint

Autophagy
Zebrafish
Regeneration
Cell Aging
Extracellular Signal-Regulated MAP Kinases
Eukaryotic Cells
Lysosomes
Mitogen-Activated Protein Kinases
Human Body
Digestion
Up-Regulation
Pharmacology
Apoptosis
Gene Expression
Wounds and Injuries

Keywords

  • autophagy
  • cell death
  • differentiation
  • regeneration
  • zebrafish

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Autophagy is required for zebrafish caudal fin regeneration. / Varga, M.; Sass, M.; Papp, D.; Takács-Vellai, K.; Kobolak, J.; Dinnyés, A.; Klionsky, D. J.; Vellai, T.

In: Cell Death and Differentiation, Vol. 21, No. 4, 04.2014, p. 547-556.

Research output: Contribution to journalArticle

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AU - Klionsky, D. J.

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